Inexpensive double-antibody fluoroimmunoassay for aminoglycoside antibiotics, phenytoin, and theophylline in serum.

We describe simple, clinically useful double-antibody fluoroimmunoassays for amikacin, gentamicin, tobramycin, theophylline, and phenytoin. The fluorescent tracers were prepared by conjugation to fluorescein isothiocyanate; the antisera were raised in rabbits. A simple filter fluorometer and disposable culture tubes are used.