Photodynamic therapy with chlorin e(6) for skin metastases of melanoma.

Background: Photodynamic therapy (PDT) has been successfully applied in clinical settings to destroy neoplasms, but the efficacy of such a treatment is dependent on the type of neoplasm and the photosynthesizer used. Here, we perform a clinical assessment of PDT for skin metastases of pigmented melanoma using chlorin e(6).

Study Design/materials And Methods: PDT with chlorin e(6) photosensitizer was administered to 14 patients with skin metastases from melanoma (10 females, four males, mean age 49.

[Singlet oxygen and photodestruction of erythrocyte membranes, sensitized by chlorin e6].

By using scavengers of active oxygen forms (AOF) the role of 1O2, O2- and HO in photodestruction of protein and lipid components of erythrocyte membranes and also in photohemolysis of erythrocytes sensitized by chlorine e 6 were studied. It was found that 1O2 plays the dominant role in these processes, but contribution of other AOF, O2- in particular, can not be fully excluded.

[Characteristics of complex-formation of chlorine e6 with human and bovine serum albumins].

Pigment-protein complexes of chlorin e6 (Chl e6) with human (HSA) and bovine serum albumines (BSA) have been investigated by spectral-luminescent methods. Fluorescence quenching of tryptophan residues caused by the inductive-resonance energy transfer to pigment molecules and the rise of the polarization degree of Chl e6 emission were observed upon incorporation of Chl e6 in the protein globula. The obtained data on spectral-energetic parameters of protein tryptophanyls and Chl e6 permitted us to calculate the energy transfer critical distances R0 in complexes of Chl e6 with HSA (R0 = 32 A) and BSA (R0 = 35A).

[Photodynamic effect of chlorine e6 on erythrocyte membranes].

Photodestruction of erythrocyte membranes sensitized by water soluble chlorophyll derivative chlorine e6 (Chl e6) was studied. It has been determined that light irradiation of erythrocyte ghosts with wave length lambda-660 nm in the presence of Chl e6 caused in protein and lipid components of the membrane deep destructive changes which were expressed in cross-linking of the membrane polypeptides and accumulating in the membrane products of peroxidation of unsaturated fatty acids residues, in phospholipids mainly. It has been shown that these processes were realized only in the presence of molecular oxygen and consequently possess pure photodynamic character.

[Erythrocyte photohemolysis sensitized by chlorin e6].

Hemolysis of human erythrocytes photosensitized by chlorophyll derivative--chlorin e6 has been studied. This process was found to be oxygen-dependent. High photodynamic activity of chlorin e6 is explained both by its specific binding with cells and by its photophysics parameters.