[Development of a method of obtaining lyophilized antileukocyte histotyping sera intended for long-term storage].

An optimal scheme for sublimation of anti-HLA-sera of locuses A, B, C, and DR has been developed, consisting of freezing at 60 degrees C and lyophilization to 29-30 degrees C, this permitting the preparation of initially active antileukocytic sera. The activity and specificity of defrosted native and lyophilized anti-HLA-sera were assessed in the complement-dependent cytotoxicity test. Four-year follow-up of the activity of dried anti-HLA-sera showed that the stabilizer was not needed, for the activities of the sera lyophilized with and without it was virtually equally high.

[The characteristics of CD18, CD11b, CD4, CD8 and CD3 antigen expression detectable by ICO monoclonal antibodies in the blood of hemoblastosis patients].

CD18-positive cells (beta-chain of antigen LFA-1), cells CD11b+, CD4+, CD8+ and CD3+ were quantified in the blood of 32 acute leukemia patients, 38 chronic lymphoid leukemia patients and 15 mature-cell lymphosarcoma patients. A standard reaction of indirect immunofluorescence was employed using ICO monoclonal antibodies. Alterations in the content and in the proportions of the main effector populations in the peripheral blood of hemoblastosis patients were observed.

[Effect of the pH of the medium on the growth of a carminomycin producer and on the biosynthesis of the carminomycin complex].

The effect of the nutrient medium acidity on the growth of the carminomycin-producing organism and formation of the carminomycin complex with its aid was studied. Satisfactory development of the organism was observed when pH of the nutrient medium on the culture inoculation was 4.9 to 8.