Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips.

Colorectal cancer (CRC) is the third most common malignancy in industrialized countries. Despite the advances in diagnostics and development of new drugs, the 5-year survival remains only 60-65%. Our approach to early diagnostics of CRC is based on the determination of serological signatures with an array of hemispherical hydrogel cells containing immobilized proteins and oligosaccharides (glycochip).

[Proteomic methods of protein separation and analysis].

The review briefly presents principles and stages of execution of such highly effective methods of protein separation as capillary, 2D gel-electrophoresis, liquid chromatography, the possibility of their successful combination with tandem mass-spectrometry and application in various fields of proteomics. The main problems of proteomic analysis as well as ways of solving by using mass-spectrometry are examined. 2 main approaches during protein identification are described in the review, characteristics and possibilities of various top-down and bottom-up proteomic analytical programs are provided.

[Mass spectrometry in medicine and biotechnology].

History of development and improvement of tandem mass spectrometry, possibilities of its application at the contemporary stage in various fields of medicine and biotechnology including production of novel medicinal preparations, identification of biologically active substances, pathogenic microorganisms and causative agents of especially dangerous infections is given.

[Molecular genetic echovirus 9 variants identified in patients with aseptic meningitis in Russia in 2007-2009].

In 2009 echovirus 9 caused a higher seasonal incidence of enterovirus infection (EVI) and its several outbreaks in a number of regions of Russia. Analysis of the partial VP1 coding region differentiated 4 phylogenetic lineages of echoviruses 9 variants identified in patients with aseptic meningitis and EVI in 2007-2009. One variant of echovirus 9 was most commonly encountered in 2009.