[Immobilized proteases and their activators as immunocorrective agents].

The influence of immobilized proteases such as catalytin (a complex preparation containing the protease terrilytin and the antiseptic catapol) and streptodecase (an activator of fibrinolysis proteases) on the humoral immune response induced by the thymus-dependent antigen (SRBC) under conditions of burns and Staphylococcus infected burns was studied. The immobilized proteases catalytin and streptodecase had an immunostimulating effect under conditions of extended burns. Catalytin had an immunocorrigating effect under conditions of the infected burns.

[The effect of tetrachloromethane and ethanol on the release by rat splenocytes of immunomodulating factors].

In combined introduction tetrachloromethane (TCM) and ethanol the spleen cells secrete both immunostimulating agent with a molecular mass (MM) of 10 - 25 kD and immunosuppressing agents with MM > 100 kD. Sticking and nonsticking to the glass splenocytes of the animal affected by TCM and ethanol, excrete, respectively, the immunostimulating or immunosuppressing agents to the supernatant liquid of the splenocytes.

[Extracorporeal immunostimulating effect of terrilytin and lysozyme in vibration injury].

In injection of allogeneic erythrocytes treated with terrilytin of lysozyme (LTE) the most marked effect was produced by LTE injection in vibration-exposed rats (VER). When the VER were given injections of various fractions (light, moderate, and heavy) of erythrocytes previously incubated with enzymes, the heavy LTE possessed a marked immunostimulating effect. However, the heavy erythrocyte fraction proved to cause a stronger effect when it was treated successively with terrilytin and lysozyme.

[Protease inhibitors as immunomodulators in experimental acute pancreatitis and staphylococcal infection].

The influence of protease-inhibiting preparations on the development of humoral immune response in diseases involving the development of secondary immunodeficiency (experimentally induced acute pancreatitis and staphylococcal infection) has been studied. Five injections of contrycal and epsilon-aminocaproic acid (epsilon-ACA), starting from day 1 after the induction of acute pancreatitis, normalized the immune response induced by sheep red blood cells 24 hours after operation. In staphylococcal infection protease-inhibiting preparations (contrycal, epsilon-ACA, Amben) produced a protective effect, increasing the survival rate and the mean survival time of the animals infected with staphylococci.

[Extracorporeal immunostimulating effect of terrilytin and lysozyme].

The role of splenocytes and erythrocytes in showing an extracorporal action by terrilytin and lysozyme was studied. The extracorporal effect of terrilytin was to a greater extent mediated by the spleen cells adhering to the plastic while the extracorporal effect of lysozyme was mainly mediated by the heavy ("old") erythrocytes. The heat treatment at a temperature of 42 degrees C for 15 minutes did not abolish the terrilytin extracorporal effect mediated by the erythrocytes but completely abolish the similar effect induced by lysozyme which bound to the erythrocyte membrane.