Colonization by Staphylococcus aureus of Nano-Structured Fluorinated Surfaces, Formed by Different Methods of Ion-Plasma Technology.

Colonization of fluorinated surfaces produced by ion-plasma technology by Staphylococcus aureus was studied by scanning electron microscopy and surface energy analysis. It was shown that the intensity of colonization was determined by the surface relief and fluorine content. Formation of nanostructured surfaces accompanied by a sharp decrease in the surface energy prevented adhesion of Staphylococcus aureus cells to the fluorine-containing surface.

[EXPERIMENTAL RATIONALE OF ENDODONTIC THERAPY OF CHRONIC FORMS OF PULPITIS- AND PERIODONTITIS USING PHOTO-ACTIVATED DISINFECTION AND ULTRASOUND TREATMENT].

Aim: Laboratory rationale of application of a complex effect of ultrasound treatment, and photo-activated disinfection in experiments in vitro using strains of anaerobic and microaerophilic microorganisms isolated from patients with chronic forms of pulpitis and periodontitis.

Materials And Methods: Microbiologic study of root canal was carried out using a bacteriologic method (2 species of obligatory-anaerobic and 2 -microaerophilic streptococci). Evaluation of the effect of ultrasound treatment in a complexwith a system of photo-activated disinfection on microflora of root canal was carried out using a bioreactor.

[STUDY OF COLONIZATION PROCESSES AND PERSISTENCE OF MICROORGANISMS IN ARTIFICIAL MATERIALS FOR MEDICAL USE].

Aim: Study processes of microbial colonization and persistence of microorganisms in polymer materials for medical use.

Materials And Methods: Samples (1 x 1 cm plates) of polymer plastics for production of removable dental prosthesis based on polyurethane and acryl were used, that were incubated with clinical isolates of Pseudomonas aeuruginosa, Staphylococcus aureus in Luria-Bertani broth nutrient media for 24, 48 hours and 7, 14 days and for 1, 5 and 3 months at a temperature of 37 degrees C. Dynamics of interaction process of microorganisms with polymer materials were studied using scanning electron microscope Quanta 200 3D (FEI Company, USA).

[A role of long-term stress and functional properties of the endothelium in the development of autonomic dystonia syndrome].

Objective: To study a role of long-term stress and endothelial function in the development of autonomic dystonia syndrome (ADS) in young patients.

Material And Methods: Authors examined 75 male patients, aged from 17 to 28 years, with ADS including 41 patients without clinically evident symptoms of psycho-emotional stress (group I) and 34 patients with high level of psycho-emotional stress (group II). A comparison group consisted of 21 healthy volunteers.

The law of stage-by-stage development of human tumors, derived from the results of ploidometric studies.

The ploidy of 56,000 epithelial cell nuclei was studied at different stages of carcinogenesis by Imager-CG computer image analyzer. The linear relationship between carcinogenesis stages and quantitative parameters (indexes of mean ploidy of cell nuclei) at a certain stage was detected and mathematically described. A step-by-step increase of DNA content in cell nuclei by one unit of ploidy leads to emergence of new biological properties, measured by the number of units surpassing the normal cell nuclear ploidy values, which was called "the malignancy code".