Correction for 'A novel electrochemical sensor based on microporous polymeric nanospheres for measuring peroxynitrite anion released by living cells and studying the synergistic effect of antioxidants' by Fuxin Liu , , 2019, , 6905-6913, https://doi.org/10.1039/C9AN01693G.
View Article and Find Full Text PDFIt is of great significance to realize the detection of trace amounts of superoxide anions (O•) in biological systems due to the connection between the concentration of O• and some diseases. Here, we present a novel method to fabricate a non-enzymatic electrochemical sensor for measuring O• from biological systems. The sensor was fabricated by nanocomposites (PAMAM-Au) synthesized by using (PAMAM-G4.
View Article and Find Full Text PDFPeroxynitrite anion (ONOO-) is a crucial reactive nitrogen species (RNS), which has aroused immense research interest in the biological and biomedical fields because aberrant expression levels of ONOO- are related to many diseases. In this work, a novel electrochemical sensor is described for the detection of peroxynitrite anion (ONOO-) released from living cells. It is constructed with a glassy carbon electrode (GCE) decorated with a nanocomposite (CTS-MPNS) synthesized from chitosan (CTS) functionalized microporous polymeric nanospheres (MPNS).
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
August 2017
The residue of the pesticides affects seriously the quality and safety of traditional Chinese medicine. Pesticide residue has caused ever-growing attention of people at home and abroad. Rapid detection techniques used for rapid screening of pesticide residues have expanded in a fast progress.
View Article and Find Full Text PDFBackground: NeuN and Nissl staining (toluidine blue, cresyl violet staining) are routinely used methods in unbiased stereological estimation of the total number of hippocampal neurons.
New Method: In the present study, we stained serial frozen coronal sections from 5 normal adult male Sprague-Dawley rat brains with different methods, measured the deformation of hippocampal area in brain sections and estimated the total number of hippocampal neurons using the optical fractionator.
Results: The deformation in x, y-axis was not obviously different with different staining protocols, but shrinkage in z-axis was significant after staining (p < 0.
The sulfate reduction-ammonia oxidation reaction with ANAMMOX sludge at autotrophic condition was implemented. It was found that the pH level decreased during the reaction. Elemental sulfur and nitrogen gas were the final products, while NO3(-) -N was the intermediate product during the sulfate reduction-ammonia oxidation reaction.
View Article and Find Full Text PDFHuan Jing Ke Xue
November 2013
The anaerobic biological transformation characteristics of sulfate and ammonia were investigated in the upflow anaerobic biofilm reactor under inorganic nutrition condition by controlling different hydraulic retention time, NH4(+)-N and SO4(2-)-S ratio and HCO3- concentration in the influent. The results showed that NH4+ and SO4(2-) were synchronously removed. The maximum NH4(+) -N and SO4(2-) -S removal rate was 47.
View Article and Find Full Text PDFObjective: To explore the effect of seminal plasma lipoprotein (a) in abnormal semen liquefaction and its clinical significance.
Methods: According to The WHO Laboratory Manual for the Examination and Processing of Human Semen, we conducted semen routine analyses of 101 patients with abnormal semen liquefaction and 26 normal healthy controls. We added chymotrypsin to the semen for 30 minutes of incubation at 37 degrees C.
Diabetic hyperglycemia causes a variety of pathological changes. Astragaloside IV (AS-IV) was widely used for the treatment of cardiovascular diseases in China. The aim of this study was to determine the effect of AS-IV on bone marrow mesenchymal stem cells (MSCs) and the underlying mechanism in diabetes.
View Article and Find Full Text PDFThe role of histamine and its receptors in basal ganglia neurocircuitry was assessed in apomorphine-induced turning behavior. Rats with unilateral 6-hydroxydopamine lesions of the substantia nigra pars compacta and medial forebrain bundle were administered histaminergic agents, and apomorphine-induced turning behavior was tested on Days 7 and 14 post-lesion. Compared with saline-treated rats, histidine (500 mg/kg, i.
View Article and Find Full Text PDFPrevious studies have suggested that brain histamine is involved in the pathogenesis of Parkinson's disease (PD), but the role of endogenous histamine in the degeneration of dopaminergic neurons in the substantia nigra pars compact (SNpc) remains unclear. We aimed to investigate this issue by changing the brain histamine levels by giving histaminergic agents, and administrating histamine receptor antagonists in the PD animal model, i.e.
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