A useful PCR primer set for the ectomycorrhizal fungus in wild pine rhizosphere based on the nuclear ribosomal DNA IGS2 sequence.

is an edible ectomycorrhizal mushroom that forms a symbiotic association with Pinaceae trees by constructing a large extraradical mycelial area (called a shiro) in the soil. The detection of this fungal mycelium in the soil is crucial for estimating the success of outplanted mycorrhizal seedlings inoculated with under experimental conditions. Although several -specific DNA markers have been reported for efficient detection in the field, no comparative study has been conducted to assess their effectiveness.