Veillonella orientalis sp. nov., an anaerobic Gram-stain-negaitve coccus isolated from saliva of a Thai child.

Objective: The genus Veillonella is one of the major and important constitutions of the oral microbiome. A novel anaerobic, Gram-negative coccus belonging to the genus Veillonella was isolated from the saliva of a child. In the present study, the characterization of strain S12025-13, is described with the comparison to established species of the genus Veillonella and a novel oral species of the genus Veillonella is proposed.

Comparative Pan-Genome Analysis of Oral Species.

The genus is a common and abundant member of the oral microbiome. It includes eight species, , , , , , , and . They possess important metabolic pathways that utilize lactate as an energy source.

Complete Genome Sequence of Veillonella nakazawae JCM 33966 (=CCUG 74597), Isolated from the Oral Cavity of Japanese Children.

We report the complete genome sequence of JCM 33966 (=CCUG 74597). This bacterium is a member of the oral and has the potential to be anticariogenic as an oral probiotic seed.

Identification and phylogenetic analysis of oral species isolated from the saliva of Japanese children.

As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral are limited. The oral community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status.

Draft Genome Sequences of Two Veillonella tobetsuensis Clinical Isolates from Intraoperative Bronchial Fluids of Elderly Patients with Pulmonary Carcinoma.

To date, has been known as an oral anaerobe and a facilitator of early-stage oral biofilm development with streptococci. Here, we report the draft genome sequences of 2 strains of first isolated from intraoperative bronchial fluids of elderly patients with pulmonary carcinoma.

Effects of chemical treatment as an adjunctive of air-abrasive debridement on restoring the surface chemical properties and cytocompatibility of experimentally contaminated titanium surfaces.

The aim of this study was to evaluate the effects of three different chemotherapeutic agents, following air-abrasive debridement, on surface chemical properties and cytocompatibility. Disks contaminated with Streptococcus gordonii biofilm were treated with air-abrasion and immersion in either 0.9% NaCl (Air + NaCl), 0.

Identification of an early stage biofilm inhibitor from Veillonella tobetsuensis.

Oral biofilm, the cause of dental caries and periodontal diseases, consists of multiple bacterial species. Streptococcus spp. and Veillonella spp.

Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK.

Recently, Veillonella infantium was isolated from tongue biofilm of a Thai child and established as a novel Veillonella species. In this study, a species-specific primer was designed to identify V. infantium on the basis of the sequence of the 70 kDa heat shock protein (dnaK) gene of Veillonella infantium JCM 31738 (= TSD-88).

Veillonella infantium sp. nov., an anaerobic, Gram-stain-negative coccus isolated from tongue biofilm of a Thai child.

A strain of a novel anaerobic, Gram-stain-negative coccus was isolated from the tongue biofilm of a Thai child. This strain was shown, at the phenotypic level and based on 16S rRNA gene sequencing, to be a member of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus Veillonella.

Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis.

Streptococcus sanguinis is frequently isolated from the blood of patients with infective endocarditis and contributes to the pathology of this disease through induction of interleukin (IL)-1β responsible for the development of the disease. However, the mechanism of IL-1β induction remains unknown. In this study, S.

Identification of Veillonella Species in the Tongue Biofilm by Using a Novel One-Step Polymerase Chain Reaction Method.

Six Veillonella species have been frequently isolated from human oral cavities including infectious sites. Recently, it was reported that diet, smoking, and possibly socioeconomic status can influence the bacterial profile in oral cavities. In addition, oral hygiene habits may also influence oral microbiota in terms of both numbers and diversity of microorganisms.

Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.

We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties. BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison. The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.

Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria.

Objective: To investigate the effect of the roselle calyx extract (RCE) (Hibiscus sabdariffa L.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria.

Methods: RCE was prepared by soaking roselle calyx powder with ethyl alcohol for 24 h at room temperature.

Draft Genome Sequence of Veillonella tobetsuensis ATCC BAA-2400T Isolated from Human Tongue Biofilm.

Here, we report the draft genome sequence of Veillonella tobetsuensis ATCC-BAA 2400(T). This bacterium has the remarkable ability to form oral biofilms. The genome is predicted to encode the necessary enzymes involved in the pathway that facilitates the conversion of lactate to propionate.

Monitoring the decontamination efficacy of the novel Poseidon-S disinfectant system in dental unit water lines.

Background: Contaminated dental unit waterlines (DUWLs) are a known source of specific health care-acquired infections because of the difficulty in keeping them clean during routine dental practice. Recently, an electrolysis apparatus that uses only the chlorine normally present in municipal water, the Poseidon-S system, was developed as a novel additive-free disinfectant system to control microbial contamination in DUWLs.

Methods: The microbiological quality of water samples collected from DUWLs was assessed before and after installation of the Poseidon-S system in terms of the total viable counts (TVCs) of microorganisms.

The interaction between Streptococcus spp. and Veillonella tobetsuensis in the early stages of oral biofilm formation.

Dental plaque is a multispecies oral biofilm, the development of which is initiated by adherence of the pioneer Streptococcus spp. Oral Veillonella spp., including V.

Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating.

Because dental implant abutments are located at transmucosal sites, their surface should inhibit bacterial accumulation to prevent peri-implantitis. The authors examined the effects of human lactoferrin (LF), an antibacterial protein present in saliva, as an antibacterial coating on the titanium surface and evaluated its effects before and after mucin-containing artificial saliva (AS) incubation. In the control group, titanium disks were soaked in distilled water, whereas in the LF group, titanium disks were soaked in LF solution to coat the disks.

The influence of oral Veillonella species on biofilms formed by Streptococcus species.

Oral Veillonella, Veillonella atypica, Veillonella denticariosi, Veillonella dispar, Veillonella parvula, Veillonella rogosae, and Veillonella tobetsuensis are known as early colonizers in oral biofilm formation. To investigate the role of oral Veillonella, biofilms formed by the co-culture of Streptococcus gordonii, Streptococcus mutans, Streptococcus salivarius, or Streptococcus sanguinis, with oral Veillonella were examined at the species level. The amount of biofilm formed by S.

Analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16S rDNA gene sequence.

Purpose: To analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16S rDNA gene sequences.

Materials And Methods: Subgingival bacterial specimens were taken from 30 patients: control (n = 15), consisting of patients with only healthy implants; and test (n = 15), consisting of patients with peri-implantitis. In both groups, subgingival bacterial specimens were taken from the deepest sites.

Identification of Veillonella tobetsuensis in tongue biofilm by using a species-specific primer pair.

Veillonella atypica, Veillonella denticariosi, Veillonella dispar, Veillonella parvula, and Veillonella rogosae have been reported to be isolated from human oral cavities. The recently detected Veillonellatobetsuensis in human tongue biofilms was proposed as a novel Veillonella sp. In this study, to determine the distribution and frequency of V.

Coaggregation between Prevotella oris and Porphyromonas gingivalis.

Background/purpose: The coaggregation of bacteria has been defined as one of the most important processes in the oral infection such as periodontitis. Prevotella oris and Porphyromonas gingivalis, which are two of the periodontopathogens, are frequently detected in severe forms of periodontal diseases. However, the interaction between P.

Lipopolysaccharide extracted from Porphyromonas gingivalis induces DNA hypermethylation of runt-related transcription factor 2 in human periodontal fibroblasts.

Background/purpose: Epigenetic alterations such as DNA methylation and histone acetylation are described as changes in the pattern of gene expression not involving the DNA sequence. Lipopolysaccharide (LPS) derived from Porphyromonas gingivalis has been shown to inhibit osteoblastic cell differentiation. We examined whether DNA hypermethylation was involved in the inhibitory effect of LPS on osteoblastic differentiation of fibroblasts derived from human periodontal ligament (HPDL).

The release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from human erythrocyte membranes lysed by hemolysin of Prevotella oris.

We found that a 38-kDa protein was released from erythrocyte membranes lysed by hemolysin of Prevotella oris, although hypotonic hemolysis did not show such a phenomenon. The 38-kDa protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by N-terminal amino acid sequencing. This study discusses the relationship between GAPDH and hemolysis.