TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4.

The high prevalence of KRAS mutations in pancreatic cancer (PC) is widely acknowledged and results in the resistance of targeted ferroptosis therapy and immunotherapy. Herein, via a CRISPR/Cas9 library screen, the effects of ferroptosis agonists were increased in KRAS-mutant PC cells upon knockout of tropomodulin 3 (TMOD3), while these effects were not observed in KRAS-wild-type cells. Increased levels of TMOD3 were found in PC tissues, particularly in those with KRAS mutations.

[Research on the trash content measurement and classification of ginned cotton by using NIR spectroscopy technique].

Near infrared (NIR) spectroscopy was investigated to predict trash content and classify types of ginned cotton by using a fiberoptic in diffuse reflectance mode. Different spectra preprocessing methods were compared, and partial least-squares (PLS) regression was established to predict the trash content of ginned cotton. Discriminant analysis (DA) was used to classify various types of lint and content level of trash.