Publications by authors named "Fusao Shimokawa"

Article Synopsis
  • The study develops a new method for assembling cells into clusters at a single-cell level using a specially designed microtool in a microfluidic device.
  • The microtool is created with antibodies that bind to specific proteins on the surface of cancer cells, enabling precise capture and assembly of the cells.
  • This platform allows for the efficient creation of cell clusters for applications in regenerative medicine and drug testing, mimicking real tissue and organ behavior.
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Article Synopsis
  • Researchers created microtools that use light to control and process individual biomolecules, featured within a microfluidic system under an optical microscope.
  • These microtools have enzymes attached that help chemically modify biomolecules in a small space, enabling precise positioning and manipulation of samples.
  • The system can effectively cut single DNA strands, demonstrating its potential for analyzing DNA at the single-molecule level and processing a variety of biological samples.
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Unlike tactile displays that use mechanical actuators, electrode-type tactile displays can be easily integrated and miniaturized because they consist of electrodes and insulators. Electrical tactile displays only require electrodes and use an electric current to stimulate vibration or pressure. Likewise, electrostatic friction tactile displays also only require electrodes and an insulator and can induce changes in friction between the display and a fingerpad.

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DNA analysis based on the observation of single DNA molecules has been a key technology in molecular biology. Several techniques for manipulating single DNA molecules have been proposed for this purpose; however, these techniques have limits on the manipulatable DNA. To overcome this, we demonstrate a method of DNA manipulation using microstructures captured by optical tweezers that allow the manipulation of a chromosomal DNA molecule.

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Optical tweezers are powerful tools for manipulating single DNA molecules using fluorescence microscopy, particularly in nanotechnology-based DNA analysis. We previously proposed a manipulation technique using microstructures driven by optical tweezers that allows the handling of single giant DNA molecules of millimetre length that cannot be manipulated by conventional techniques. To further develop this technique, the authors characterised the microstructures quantitatively from the view point of fabrication and efficiency of DNA manipulation under a fluorescence microscope.

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We propose a novel Kretschmann-type surface plasmon resonance (SPR) sensor chip having a surface covered with electrodeposited gold nanostructures to enhance the sensitivity of SPR biosensing. The nanostructure is three-dimensional and has a larger surface area than a conventional flat surface chip, which increases the amount of protein binding and also induces a large change in the effective dielectric constant of the sensing area. The gold nanostructures were formed by electrodeposition under galvanostatic conditions, so their size could be controlled by manipulating the deposition time and current.

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The electrodeposition of gold nanostructures increases the surface area of a biosensor, which brings an enhancement of the sensitivity by increasing the amount of analyte binding to the surface. To evaluate the relationship among the surface structure, the area and the analyte binding, we quantitatively analyzed them for quartz crystal microbalance (QCM) sensing by scanning electron microscopy and cyclic voltammetry measurements. The results indicate a several-times increase of analyte bindings, and also the limitation of the sensing performance.

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