Salt stress can seriously affect the growth and development of maize ( L.), resulting in a great yield loss. Melatonin (MT), an indole hormone, is a potential enhancer of plant tolerance against salt stress.
View Article and Find Full Text PDFMaize ( L.) is sensitive to salt stress, especially during seed germination and seedling morphogenesis, which limits maize growth and productivity formation. As a novel recognized plant hormone, melatonin (MT) participates in multiple growth and developmental processes and mediates biotic/abiotic stress responses, yet the effects of salt stress on maize seedlings remain unclear.
View Article and Find Full Text PDFNucleic Acids Res
October 2024
The efficient refactoring of natural product biosynthetic gene clusters (BGCs) for activating silent BGCs is a central challenge for the discovery of new bioactive natural products. Herein, we have developed a simple and robust CRISETR (CRISPR/Cas9 and RecET-mediated Refactoring) technique, combining clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 and RecET, for the multiplexed refactoring of natural product BGCs. By this approach, natural product BGCs can be refactored through the synergistic interaction between RecET-mediated efficient homologous recombination and the CRISPR/Cas9 system.
View Article and Find Full Text PDFBenzoxazinoids (BXs) are unique bioactive metabolites with protective and allelopathic properties in maize in response to diverse stresses. The production of BXs involves the fine regulations of BXs biosynthetic gene cluster (BGC). However, little is known about whether and how the expression pattern of BGC members is impacted by biotic and abiotic stresses.
View Article and Find Full Text PDFSerotonin (5-HT), an indoleamine compound, has been known to mediate many physiological responses of plants under environmental stress. The deep-seeding (≥20 cm) of maize seeds is an important cultivation strategy to ensure seedling emergence and survival under drought stress. However, the role of 5-HT in maize deep-seeding tolerance remains unexplored.
View Article and Find Full Text PDFIntroduction: The Chinese Government initiated the Donation after Citizens' Death policy in 2010. To now, it has been a major source of organs for transplant. Since it is still a young policy, corresponding clinical evidence is still urgently needed for its improvement.
View Article and Find Full Text PDFFront Bioeng Biotechnol
March 2022
Recent work has shown that deep convolutional neural network is capable of solving inverse problems in computational imaging, and recovering the stress field of the loaded object from the photoelastic fringe pattern can also be regarded as an inverse problem solving process. However, the formation of the fringe pattern is affected by the geometry of the specimen and experimental configuration. When the loaded object produces complex fringe distribution, the traditional stress analysis methods still face difficulty in unwrapping.
View Article and Find Full Text PDFDaptomycin (DAP) is a novel microbial lipopeptide antibiotic synthesized by the DAP biosynthetic gene cluster dpt of Streptomyces roseosporus (S. roseosporus). DptP gene locates upstream of dpt and confers DAP resistance to Streptomyces ambofaciens (S.
View Article and Find Full Text PDFIn this study, a simple method was proposed to calculate electrode-sample contact impedance in the cases of two-point and four-point measurements. The results indicated that when using the saturated calcium hydroxide solution (SCH) as conductive medium, the contact impedance in the four-point measurement is negligible for the impedance range of cement-based materials. The SCH can be used as a reference for correction of the contact impedance.
View Article and Find Full Text PDFBacterial cellulose (BC) has extensive application prospects in many fields in view of its unique characteristics. However, the large-scale applications of BC are severely limited because of relatively low BC productivity and high cost of culture medium. Herein, the distiller's grain enzymatic hydrolysate (DEH) and yellow water were successfully combined as an effective substitute (the best distiller's grains-yellow water medium, BDY medium) for traditional Hestrin-Schramm medium (HS medium) for BC production by Gluconacetobacter xylinus through the response surface methodology.
View Article and Find Full Text PDFThe data presented in this article are the wind measurements acquired from a tower in Southeast China during typhoon Nesat (1709#) and typhoon Haitang (1710#). Three 3D ultrasonic anemometers Wind Master Pro were utilized to obtain 3D wind data. The anemometer works well with wind speed range of 0-65 m/s and wind angle range of 0-360°.
View Article and Find Full Text PDFObjective: This study aimed to investigate the role of the Notch1/Delta-like 4 signaling pathway and its relationship with T helper 17 (Th17) cells in the peripheral transplantation immune of renal transplant recipients.
Methods: Fifty-two kidney transplant recipients in our hospital were selected and divided into the acute rejection group (AR), renal tubular necrosis (ATN) group, and stable renal function group, according to their postoperative recovery. Flow cytometry was used to detect the expression of Notch1 and Delta-like 4 in peripheral lymphocytes and the presence of Th17 cells in the kidney of transplant recipients.
The renal allograft survival rates of patients with immunoglobulin A nephropathy (IgAN), and patients with or without other glomerular diseases, have yet to be fully elucidated. In this study, the clinicopathological factors associated with long-term allograft survival for the prognosis of renal allograft recipients with IgAN were examined. All patients enrolled in this study were diagnosed with IgAN following clinical and pathological examinations.
View Article and Find Full Text PDFBackground Aims: Minimal change nephrotic syndrome is the most frequent cause of nephrotic syndrome in childhood. Current treatment regimes, which include glucocorticoid hormones and immunosuppressive therapy, are effective and have fast response. However, because of the side effects, long treatment course, poor patient compliance and relapse, novel approaches for the disease are highly desired.
View Article and Find Full Text PDFBackground: The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.
Results: Cisplatin increased GC apoptosis from 0.59% to 13.