Age differences in coping and emotional responses toward SARS: a longitudinal study of Hong Kong Chinese.

This study examined age-related emotional responses and coping at the peak and the end of the SARS outbreak in Hong Kong. Three hundred and eighty-five Hong Kong Chinese, aged 18-86 years, rated the extent that they experienced 'shock', 'sadness', 'anger' and 'fear' in the face of SARS. They also completed selected items from Brief COPE (Carver, 1997).

Toward full-sequence de novo protein design with flexible templates for human beta-defensin-2.

In this article, we introduce and apply our de novo protein design framework, which observes true backbone flexibility, to the redesign of human beta-defensin-2, a 41-residue cationic antimicrobial peptide of the innate immune system. The flexible design templates are generated using molecular dynamics simulations with both Generalized Born implicit solvation and explicit water molecules. These backbone templates were employed in addition to the x-ray crystal structure for designing human beta-defensin-2.

Fluorescence excitation spectra of the b (1)Pi(u), b(') (1)Sigma(u) (+), c(n) (1)Pi(u), and c(n) (') (1)Sigma(u) (+) states of N(2) in the 80-100 nm region.

Fluorescence excitation spectra produced through photoexcitation of N(2) using synchrotron radiation in the spectral region between 80 and 100 nm have been studied. Two broadband detectors were employed to simultaneously monitor fluorescence in the 115-320 nm and 300-700 nm regions, respectively. The peaks in the vacuum ultraviolet fluorescence excitation spectra are found to correspond to excitation of absorption transitions from the ground electronic state to the b (1)Pi(u), b(') (1)Sigma(u) (+), c(n) (1)Pi(u) (with n=4-8), c(n) (') (1)Sigma(u) (+) (with n=5-9), and c(4) (')(v('))(1)Sigma(u) (+) (with v(')=0-8) states of N(2).

Interleukin-1alpha and -1beta promoter polymorphisms in Taiwanese patients with dementia.

Background: Inflammatory events may contribute to the pathogenesis of dementia and interleukin-1 (IL-1) may exert both neurotoxic and neuroprotective effects. We investigated whether IL-1alpha -889 C/T and IL-1beta -511 C/T promoter polymorphisms are associated with the risk of Alzheimer's disease (AD) and vascular dementia (VaD).

Methods: AD patients (n = 219) and VaD patients (n = 82), who fulfilled the criteria of the NINCDS-ADRDA and NINDS-AIREN, and ethnic-matched and nondemented controls (n = 209) were analyzed by means of genotype association method.

An interactive lesson in acid/base and pro-drug chemistry using sodium gamma-hydroxybutyrate and commercial test coasters.

Objective: To develop a classroom activity that applied pertinent pharmaceutical concepts to examine the use and limitations of a commercially available test drink coaster in detecting the presence of a date-rape drug, sodium gamma-hydroxybutyrate (NaGHB), in beverages.

Design: An activity exercise involving a combination of self-study, hands on participation, and classroom discussion was developed. Topics incorporated into the activity were drug-assisted rape, the concepts of false positives and negatives, and prodrug and pH chemistry.

Deletion at ITPR1 underlies ataxia in mice and spinocerebellar ataxia 15 in humans.

We observed a severe autosomal recessive movement disorder in mice used within our laboratory. We pursued a series of experiments to define the genetic lesion underlying this disorder and to identify a cognate disease in humans with mutation at the same locus. Through linkage and sequence analysis we show here that this disorder is caused by a homozygous in-frame 18-bp deletion in Itpr1 (Itpr1(Delta18/Delta18)), encoding inositol 1,4,5-triphosphate receptor 1.

Crypt loss is a marker of clinical severity of acute gastrointestinal graft-versus-host disease.

Background: Crypt loss is a histological finding in acute gastrointestinal Graft-Versus-Host Disease (GI-GvHD) of undefined clinical significance.

Methods: Colonic crypt loss was graded in twenty-three patients treated for GI-GvHD following stem cell transplantation and then correlated with clinical parameters of disease severity and mortality.

Results: Crypt loss was present in 17/23 cases, and in 11/23 cases crypt loss was deemed severe by the presence of contiguous areas of crypt loss.

Influence and adjustment goals: sources of cultural differences in ideal affect.

Previous studies have found that in American culture high-arousal positive states (HAP) such as excitement are valued more and low-arousal positive states (LAP) such as calm are valued less than they are in Chinese culture. What specific factors account for these differences? The authors predicted that when people and cultures aimed to influence others (i.e.

Risk factors for vascular dementia: a hospital-based study in Taiwan.

Background: In Taiwan, next to Alsheimer's, vascular dementia (VD) is the second leading cause of dementia in the elderly. Few studies have examined cardiovascular risk factors and their association with VD in Taiwan.

Methods: This is a case-control study using a sampling of subjects from the outpatient memory clinics of two hospitals.

Tandem autologous stem cell transplantation for patients with primary refractory or poor risk recurrent Hodgkin lymphoma.

Unlabelled: Although autologous stem cell transplantation (ASCT) for patients with relapsed/refractory Hodgkin lymphoma (HL) appears to offer a survival advantage over conventional therapy, only approximately 25% to 35% of patients with primary progressive or poor-risk recurrent HL can achieve durable remission after ASCT, with disease progressive after transplant accounting for most of the treatment failures. We conducted a pilot study to evaluate the toxicities and efficacy of a tandem transplant approach in this subgroup of patients. Between April 1998 and March 2000, 46 patients were enrolled in the study.

A genome-wide genotyping study in patients with ischaemic stroke: initial analysis and data release.

Background: Despite evidence of a genetic role in stroke, the identification of common genetic risk factors for this devastating disorder remains problematic. We aimed to identify any common genetic variability exerting a moderate to large effect on risk of ischaemic stroke, and to generate publicly available genome-wide genotype data to facilitate others doing the same.

Methods: We applied a genome-wide high-density single-nucleotide-polymorphism (SNP) genotyping approach to a cohort of samples with and without ischaemic stroke (n=278 and 275, respectively), and did an association analysis adjusted for known confounders in a final cohort of 249 cases and 268 controls.

Genome-wide genotyping in amyotrophic lateral sclerosis and neurologically normal controls: first stage analysis and public release of data.

Background: The cause of sporadic ALS is currently unknown. Despite evidence for a role for genetics, no common genetic variants have been unequivocally linked to sporadic ALS. We sought to identify genetic variants associated with an increased or decreased risk for developing ALS in a cohort of American sporadic cases.

Age differences in the sixth personality factor: age differences in interpersonal relatedness among Canadians and Hong Kong Chinese.

Cross-cultural studies on personality in which a combined etic-emic approach has been used have found a 6th personality factor--Interpersonal Relatedness--in addition to the usual Big Five. The authors examined age differences in Interpersonal Relatedness among Canadians and Hong Kong Chinese after statistically controlling for the Big Five. Age differences in some aspects of Interpersonal Relatedness were found among Hong Kong Chinese but not among Canadians, suggesting that development of some personality characteristics may differ across cultures.

Ovarian cycle of the captive formosan gem-faced civets (Paguma larvata taivana).

Formosan gem-faced civets are classified to be endemic sub-species of Paguma larvata in Taiwan. Little about their reproductive physiology has been reported. This study was designed to characterize the ovarian activity throughout the year and define ovarian cycle length and the lengths of its component phases.

Association of tau haplotype-tagging polymorphisms with Parkinson's disease in diverse ethnic Parkinson's disease cohorts.

Background: The overlap in the clinical and pathological features of tauopathies and synucleinopathies raises the possibility that the tau protein may be important in Parkinson's disease (PD) pathogenesis. Several MAPT polymorphisms that define the tau H1 haplotype have been investigated for an association with PD with conflicting results; however, two meta-analyses support an association between haplotype H1 and PD.

Methods: In this study, we recruited 508 patients and 611 healthy controls from Greek, Finnish and Taiwanese populations.

A common genetic factor for Parkinson disease in ethnic Chinese population in Taiwan.

Background: Parkinson's disease (PD) is the most common neurodegenerative movement disorder, characterized clinically by resting tremor, bradykinesia, postural instability and rigidity. The prevalence of PD is approximately 2% of the population over 65 years of age and 1.7 million PD patients (age > or = 55 years) live in China.

Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.

Background: A new locus for amyotrophic lateral sclerosis--frontotemporal dementia (ALS-FTD) has recently been ascribed to chromosome 9p.

Methods: We identified chromosome 9p segregating haplotypes within two families with ALS-FTD (F476 and F2) and undertook mutational screening of candidate genes within this locus.

Results: Candidate gene sequencing at this locus revealed the presence of a disease segregating stop mutation (Q342X) in the intraflagellar transport 74 (IFT74) gene in family 476 (F476), but no mutation was detected within IFT74 in family 2 (F2).

Gender differences in social network characteristics and psychological well-being among Hong Kong Chinese: the role of future time perspective and adherence to Renqing.

Gender differences in social network characteristics are well documented in the literature. Socio-emotional selectivity theory emphasizes the importance of future time perception on selection of social partners whereas cultural studies stress the roles of Renqing (relationship orientation) on social interactions. This study examined the effects of future time perspective and adherence to Renqing on social network characteristics, and their associations with psychological well-being of 321 Chinese men and women, aged 28-91 years.

Genome-wide SNP assay reveals structural genomic variation, extended homozygosity and cell-line induced alterations in normal individuals.

The recent hapmap effort has placed focus on the application of genome-wide SNP analysis to assess the contribution of genetic variability, particularly SNPs, to traits such as disease. Here, we describe the utility of genome-wide SNP analysis in the direct detection of extended homozygosity and structural genomic variation. We use this approach to assess the frequency of genomic alterations resulting from the lymphoblast immortalization and culture processes commonly used in cell repositories.

Genome-wide genotyping in Parkinson's disease and neurologically normal controls: first stage analysis and public release of data.

Background: Several genes underlying rare monogenic forms of Parkinson's disease have been identified over the past decade. Despite evidence for a role for genetics in sporadic Parkinson's disease, few common genetic variants have been unequivocally linked to this disorder. We sought to identify any common genetic variability exerting a large effect in risk for Parkinson's disease in a population cohort and to produce publicly available genome-wide genotype data that can be openly mined by interested researchers and readily augmented by genotyping of additional repository subjects.

Untangling the tau gene association with neurodegenerative disorders.

Pathological tau protein inclusions have long been recognized to define the diverse range of neurodegenerative disorders called the tauopathies, which include Alzheimer's disease (AD), progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration. Mutations in the tau gene, MAPT, cause familial frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17), and common variation in MAPT is strongly associated with the risk of PSP, corticobasal degeneration and, to a lesser extent, AD and Parkinson's disease (PD), implicating the involvement of tau in common neurodegenerative pathway(s). This review will discuss recent work towards the unravelling of the functional basis of this MAPT gene association.

Tigecycline: a glycylcycline antimicrobial agent.

Background: Tigecycline, the first glycylcycline to be approved by the US Food and Drug Administration, is a structural analogue of minocycline that was designed to avoid tetracycline resistance mediated by ribosomal protection and drug efflux. It is indicated for the treatment of complicated skin and skin-structure infections and complicated intra-abdominal infections and is available for intravenous administration only.

Objective: This article summarizes the in vitro and in vivo activities and pharmacologic and pharmacokinetic properties of tigecycline, and reviews its clinical efficacy and tolerability profile.

Does follicularity in large cell lymphoma predict outcome after autologous stem cell transplantation?

The purpose of this study was to evaluate whether follicular histology in large cell lymphoma influences treatment outcomes after autologous stem cell transplantation (ASCT). It remains an area of controversy whether the natural history of follicular large cell lymphoma (FLCL) is akin to diffuse large cell lymphoma (DLCL) with curative potential or is more similar to indolent follicular lymphomas with a pattern of late relapses after intensive chemotherapy. Although ASCT is a potentially curative treatment for patients with recurrent DLCL, the effectiveness of this approach in patients with FLCL is unclear.

Simultaneous determination of nitroglycerin and dinitrate metabolites in metabolism studies using liquid chromatography-mass spectrometry with electrospray ionization.

We have developed a liquid chromatographic-mass spectrometric method for the simultaneous determination of nitroglycerin (NTG) and its active metabolites, glyceryl 1,2-dinitrate (1,2-GDN) and glyceryl 1,3-dinitrate (1,3-GDN), for metabolism studies in cell cultures. 1,2,4-Butanetriol-1,4-dinitrate was chosen as an internal standard. Using a linear gradient of water/methanol containing 0.