Publications by authors named "Fumiya Ikeda"
Extremophiles
December 2024
Article Synopsis
- The novel esterase gene lipP1, found in the extremely halophilic archaeon Haloarcula japonica, encodes the enzyme HjEstP1, classified as a family IV esterase with specific conserved motifs.
- HjEstP1 was expressed in its original strain, purified, and characterized, revealing optimal activity at a pH of 6.0 and temperatures above 60 °C.
- The enzyme demonstrated increased activity with higher NaCl concentrations, peaking at over 4.5 M, and preferentially hydrolyzed pNP and glycerol esters with short-chain fatty acids, marking it as the first esterase identified from an extremely halophilic archaeon through homologous expression.
