Development and application of serum cholinesterase activity measurement using benzoylthiocholine iodide.

Background: Measurement of cholinesterase activity in serum is important to identify substantial liver disease and damage by pesticides, and to assess the degree of development of fatty liver and preoperative risk. Many procedures using various artificial substrates have been developed but suffer from problems with substrate specificity and interference by endogenous substances.

Methods: An assay pseudocholinesterase (ChE, EC 3.