Excretion of N(1), N(12)-diacetylspermine in the urine of healthy individuals.

Background: Urinary N(1),N(12)-diacetylspermine (DiAcSpm) is a novel tumour marker that can be used to detect early cancers. In this study, we examined whether spot urine samples could represent the daily excretion of DiAcSpm after creatinine normalization and which factors should be taken into account in determining reference values for this biomarker.

Methods: We collected the following urine samples: (1) samples from seven healthy volunteers collected on each day of two 2-day sessions to examine the circadian variation of DiAcSpm excretion; (2) samples from 3952 male and 1782 female volunteers to estimate the DiAcSpm concentrations in apparently healthy adults and (3) samples from 16 female volunteers collected every morning over a 3-month period to examine the menstruation-related variation in DiAcSpm excretion.

Thoracoscopic removal of a papillary fibroelastoma in the left ventricular apex.

Primary cardiac tumors located deep in the left ventricle present a surgical challenge. A mobile tumor located in the left ventricular apex was incidentally discovered on echocardiography in an 81-year-old female. The tumor was removed using a combined sternotomy and chest-port approach assisted by thoracoscopy.