Astrocyte culture on nitrocellulose membranes and plastic: detection of cytoskeletal proteins and mRNAs by immunocytochemistry and in situ hybridization.

Neonatal rat brain astrocyte secondary cultures were established on nitrocellulose membrane filters (13-mm diameter Millipore disk) and on plastic coverslips in serum-supplemented medium. On these substrata, cultured astrocytes changed their shape from flat and polygonal to stellate in the absence of hormones or growth factor supplements. Cultures became confluent after 4 days, and astrocytes on nitrocellulose filters continued to differentiate morphologically and biochemically, as evidenced by extensive cytoplasmic process formation and glial fibrillary acid protein (GFAP) accumulation.

Clearance of myelin basic protein from blood of normal and EAE rabbits.

The rate of clearance of porcine myelin basic protein (MBP) from plasma of rabbits was determined following intravenous injection of 20 mg MBP. The MBP level in the plasma was measured by a 2-site immunoradiometric assay with specific antibody to guinea pig MBP produced in rabbits. Plasma MBP-antibody levels were determined by competitive binding radioimmune assay (RIA).

Intravenous administration of fat emulsions; metabolic and clinical studies.

Fat emulsions alone or in alternation with amino acid mixtures were administered by continuous intravenous infusion to human subjects. Adequate nutrition was maintained thereby, without untoward effects. Upon too rapid administration of such emulsions, toxic manifestations occurred, apparently referable to an "overload" of the enzyme systems concerned with fat catabolism and storage.