Value of Western blotting in the clinical follow-up of canine leishmaniasis.

Specific serum antibody levels in Leishmania infantum-infected dogs treated with a combination of glucantime and allopurinol were estimated by indirect immunofluorescence and Western blotting. The sensitivity of Western blot was greater than that obtained with immunofluorescence titration. In general, both diagnostic methods concurred with the post-treatment clinical status of the animals.

Infection-induced protection against Haemonchus contortus in merino and manchego sheep. Relationship to serum antibody response.

Resistance to primary and secondary infections with Haemonchus contortus was studied in 10-month-old Manchego and Merino sheep. No notable interbreed differences were observed after primary infections in the parameters determined (prepatency period, faecal egg output, abomasal worm burden). Previously infected sheep (200 L-3/kg live weight (lw)) from both breeds showed notable protection after challenge (400 L-3/kg lw), evidenced by lower eggs/g faeces (epg) values and worm burdens.

The structure of a glycogen phosphorylase glucopyranose spirohydantoin complex at 1.8 A resolution and 100 K: the role of the water structure and its contribution to binding.

A glucopyranose spirohydantoin (a pyranose analogue of the potent herbicide, hydantocidin) has been identified as the highest affinity glucose analogue inhibitor of glycogen phosphorylase b (GPb). In order to elucidate the structural features that contribute to the binding, the structures of GPb in the native T state conformation and in complex with glucopyranose spirohydantoin have been determined at 100 K to 2.0 A and 1.

Preparation of a polypyrrole electrode modified with a nickel phthalocyanine complex. Application to the determination of an antioxidant (propylgallate) in foods.

In this paper a study of the electrocatalytic oxidation of propylgallate (PG) by using a polypyrrole electrode modified with tetrasulfonate nickel (II) phthalocyanine complex (Pt/PPy/NiPcTs) was carried out. Several parameters of this polymeric electrode, such as: (a) its stability in hydroorganic solution, by using an indium tin oxide (ITO) electrode and spectrophotometry; and (b) its thickness using electrochemical techniques, have been studied. In addition, several parameters affecting the PG voltammetric peak, such as pH, methanol water ratio, potential scan rate and analyte concentration, were considered.

Determination of aceclofenac using adsorptive stripping voltammetric techniques on conventional and surfactant chemically modified carbon paste electrodes.

An electroanalytical method for determination of the anti-inflammatory agent aceclofenac at the ppb level using adsorptive stripping voltammetric techniques on conventional and surfactant chemically modified electrodes is developed. In this electroanalytical study a process of aceclofenac adsorption on carbon particles has been identified. In order to improve the aceclofenac determination, a chemical modification of the carbon paste was carried out employing several hydrophobic substances such as phospholipid and fatty acids, as well as several surfactants such as the non-ionic Triton X-100 and Triton X-405 and anionic sodium dodecyl sulfate.

Cross antigenicity among ovine trichostrongyloidea. Preliminary report.

A preliminary trial on the extent of cross-antigenicity among the sheep strongylids Haemonchus contortus, Trichostrongylus colubriformis, Teladorsagia circumcincta and Nematodirus battus in 2.5- to 4-month-old lambs has been carried out using ELISA and Western blotting (WB). Cross antigenicity was tested using soluble extracts from adult and third stage larvae (L3) of H.

Giant dermatofibroma. A little-known clinical variant of dermatofibroma.

Background: Dermatofibroma is a common cutaneous lesion that usually appears as a slow-growing firm dermal nodule.

Objective: Our purpose was to report the clinical and histopathologic characteristics of eight giant dermatofibromas and review the few cases of this variant of dermatofibroma reported in the literature.

Methods: All clinical records of two Departments of Dermatology of University Hospitals from the past 5 years with a diagnosis of dermatofibroma were reviewed.

Voltammetric determination of piroxicam in micellar media by using conventional and surfactant chemically modified carbon paste electrodes.

A method for piroxicam determination based on adsorptive stripping voltammetric techniques, using conventional and chemically modified carbon paste electrodes in micellar media, is described. The employed surfactants were sodium dodecyl sulfate (SDS), Triton X-100, Triton X-405, Tween 80 and Brij 30. However, the purpose of this paper is, at present, to research the use of surfactants as carbon paste modifier because one of the mechanisms of hydrophobic drugs ad-accumulation on the carbon paste electrode is based on the chemical affinity.

Lamb serum recognition of infective larvae and adult Haemonchus contortus antigens.

Sixteen- to eighteen-week-old lambs were infected with 2500 3rd stage larvae (L-3) of Haemonchus contortus or kept as uninfected controls. Two months later all animals were challenged with 5000 L-3 of this parasite. Soluble antigens of H.

Effect of subclinical coccidiosis in kids on subsequent trichostrongylid infection after weaning.

Seven-week-old female kids of the Murciana-Granadina breed naturally infected with coccidia were superinfected with a multispecific Eimeria inoculum (300,000 oocysts) or treated to control the coccidial infection with Amprolium (50 mg kg-1 liveweight day-1; 4 days every 14 days); 80 days later both animal groups received 2500 third-stage larvae of a sheep-derived mixture of Trichostrongylus colubriformis (50%), Teladorsagia circumcincta (40%) and Haemonchus contortus (10%). Kids experimentally superinfected with Eimeria showed reduced food intake at the early patency period, higher numbers of oocysts passed at weaning time and lower liveweight gain than the Amprolium-medicated kids. In the Eimeria superinfected kids the trichostrongyle infection caused a tendency to result in chronic coccidial infections.

Localized familial redundant scalp: atypical cutis verticis gyrata?

Two patients, a mother and son, with a conspicuous redundant unique scalp fold are reported. No underlying disorder was found. Light microscopy was unremarkable.

A quantitative study of Eimeria infections of goats from central Spain.

A survey on coccidial infections in goats from central Spain was carried out. Fifty-five goat farms from 28 localities belonging to the three bioclimatic subregions of the area were visited. Individual samples (702) were obtained from the rectum of 130 young (under 1 year of age) and adult (572 over 1 year of age) goats.

Reiter's syndrome and human immunodeficiency virus infection: case report and review of the literature.

The concurrence of Reiter's syndrome and human immunodeficiency virus infection has recently been noted. In this report the evolution of Reiter's syndrome (arthritis, urethritis, and conjunctivitis) in a patient with a history of intravenous drug abuse and positive results of serologic tests for human immunodeficiency virus is described and the literature is reviewed.