STAT3 promotes NLRP3 inflammasome activation by mediating NLRP3 mitochondrial translocation.

Recognition of the translocation of NLRP3 to various organelles has provided new insights for understanding how the NLRP3 inflammasome is activated by different stimuli. Mitochondria have already been demonstrated to be the site of NLRP3 inflammasome activation, and the latest research suggests that NLRP3 is first recruited to mitochondria, then disassociated, and subsequently recruited to the Golgi network. Although some mitochondrial factors have been found to contribute to the recruitment of NLRP3 to mitochondria, the detailed process of NLRP3 mitochondrial translocation remains unclear.

Inverse fitting direct absorption spectroscopy Technology: Simplified implementation and enhanced performance.

The conventional direct absorption spectroscopy (DAS) technique has been plagued by the difficulty of obtaining accurate baseline, which is caused by photoelectric drift and the absence of non-absorbing regions in the transmitted light intensity signal. An inverse fitting direct absorption spectroscopy (IF-DAS) technique has been proposed to address this difficulty. The technique leverages the intrinsic nonlinear intensity response of tunable lasers to achieve baseline-free concentration measurements.

TT-type resonator-based differential photoacoustic spectroscopy for trace gas detection.

A novel TT-type resonator was proposed for the first time, to our knowledge, to realize differential photoacoustic (PA) detection for trace gas measurement. The special design of the TT-type resonator allows us to install the microphone at the resonant center of the acoustic field to maximize the use of the absorption-induced PA signal. To meet the requirement of low gas consumption and easy integration, the TT-type resonator-based PA cell was fabricated as a fiber-coupled module with an inner volume of only 1.

Acetylation is required for full activation of the NLRP3 inflammasome.

Full activation of the NLRP3 inflammasome needs two sequential signals: a priming signal, followed by a second, assembly signal. Several studies have shown that the two signals trigger post-translational modification (PTM) of NLRP3, affecting activity of the inflammasome, however, the PTMs induced by the second signal are less well characterized. Here, we show that the assembly signal involves acetylation of NLRP3 at lysine 24, which is important for the oligomerization and the actual assembly of NLRP3 without affecting its recruitment to dispersed trans-Golgi network (dTGN).

Colorimetric and photothermal dual-mode aptasensor with redox cycling amplification for the detection of ochratoxin A in corn samples.

Ochratoxin A (OTA) detection is critical for public health safety. This study proposes a G-quadruplex-Hemin/iodide (G4-Hemin/I)-mediated non-enzyme redox cycling amplification (RCA) system for dual-modal (colorimetric and photothermal thermometer) OTA analysis. The proposed aptasensor platform for point-of-care testing employs a common thermometer for quantitative signal readouts.

Simultaneous detection of greenhouse gases CH and CO based on a dual differential photoacoustic spectroscopy system.

In addition to the atmospheric measurement, detection of dissolved carbon oxides and hydrocarbons in a water region is also an important aspect of greenhouse gas monitoring, such as CH and CO. The first step of measuring dissolved gases is the separation process of water and gases. However, slow degassing efficiency is a big challenge which requires the gas detection technology itself with low gas consumption.

Application of surface-enhanced Raman scattering to qualitative and quantitative analysis of arsenic species.

Given the toxicity of arsenic, there is an urgent need for the development of efficient and reliable detection systems. Raman spectroscopy, a powerful tool for material characterization and analysis, can be used to explore the properties of a wide range of different materials. Surface-enhanced Raman spectroscopy (SERS) can detect low concentrations of chemicals.

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction.

Accurate and sensitive analysis of p53 DNA is important for early diagnosis of cancer. In this work, a fluorescence sensing system based on DNA supersandwich nanowires and cation exchange (CX)-triggered multiplex signal amplification was constructed for the detection of p53 DNA. In the presence of p53 DNA, the DNA self-assembles to form a DNA supersandwich nanowire that generates long double-stranded DNA.

Insight into biofilm formation of wastewater treatment processes: Nitrogen removal performance and biological mechanisms.

Biofilm formation affects biological nitrogen (N) removal, and a sequencing batch biofilm reactor (SBBR) was set up to evaluate the changes in N removal and microbial characteristics during biofilm formation. The results indicated that the average effluent concentration of chemical oxygen demand (COD), ammonia nitrogen (NH-N) and total nitrogen (TN) in the SBBR were 27.48, 1.

Ultra-Stable Temperature Controller-Based Laser Wavelength Locking for Improvement in WMS Methane Detection.

In the wavelength modulation spectroscopy (WMS) gas detection system, the laser diode is usually stabilized at a constant temperature and driven by current injection. So, a high-precision temperature controller is indispensable in every WMS system. To eliminate wavelength drift influence and improve detection sensitivity and response speed, laser wavelength sometimes needs to be locked at the gas absorption center.

Changes of nitrogen and phosphorus removal pattern caused by alternating aerobic/anoxia from the perspective of microbial characteristics.

The purpose of this study was to compare the impact of different numbers of alternating aerobic/anoxic (A/O) cycles on pollutant removal. Three sequential batch reactors (SBRs) with varying numbers of alternating A/O cycles were established. Under the tertiary anoxic operating conditions, the removal efficiencies of chemical oxygen demand (COD), ammonia nitrogen (NH-N), total nitrogen (TN), and total phosphorus (TP) were 88.

Intrafollicular Retinoic Acid Signaling Is Important for Luteinizing Hormone-Induced Oocyte Meiotic Resumption.

It has been clear that retinoic acid (RA), the most active vitamin A (VA) derivative, plays a central role in governing oocyte meiosis initiation. However, it has not been functionally determined if RA participates in luteinizing hormone (LH)-induced resumption from long-lasting oocyte meiotic arrest, which is essential for haploid oocyte formation. In the present study, using well-established in vivo and in vitro models, we identified that intrafollicular RA signaling is important for normal oocyte meiotic resumption.

Advanced nitrogen removal from low carbon nitrogen ratio domestic sewage via continuous plug-flow anaerobic/oxic/anoxic system: Enhanced by endogenous denitrification.

An anaerobic/oxic/anoxic continuous plug-flow biorereactor was established to derive stable advanced nitrogen removal of oligotrophic domestic wastewater by setting a sludge dual-reflux system and a mixed liquid cross-flow system, while extending the hydraulic retention time in anoxic section. The effluent total inorganic nitrogen was 7.9 ± 2.

CH/CH dual gas sensing system using a single mid-infrared laser.

Methane (CH) and ethane (CH) dual gas sensor with low system complexity and strong stability is proposed. The correction method based on absorbance spectrum is applied, and the cross-interference of CH to CH is eliminated. In the single gas concentration measurement, linear fitting is performed between the absorbance and concentration of CH and CH, and the correlation coefficients of R = 0.

[Characteristics and Planting Safety Assessment of As Content in Dryland Soil and Maize in Guizhou Province].

In order to understand the distribution characteristics of As content in dryland soils and maize seeds in Guizhou province and to evaluate the safety of maize cultivation, 468 natural soil samples, 1260 dryland surface soil samples, and 980 corresponding maize seed samples were systematically collected to determine their As content and basic physicochemical properties. The degree of contamination of the samples was evaluated by using the single-factor contamination index method. The results showed that: ① the range of (As) in dryland soil was 0.

Detection and analysis of microplastics in offshore sediment by microscopic differential Raman spectroscopy.

In view of the problems of low pre-screening efficiency, a weak Raman signal, and strong fluorescence interference in the detection of microplastics by traditional Raman spectroscopy, a set of rapid detection system and research methods for microplastics, including fluorescence imaging technology, differential Raman spectroscopy technology, and confocal microscopic Raman technology, are constructed in this paper. A 784/785 nm dual-wavelength laser was used as the excitation light source in the rapid detection system for microplastics. The sediment in the coastal waters of Qingdao Shilaoren was taken as the research object.

Feature Domain Transform Filter for the Removal of Inherent Noise Bound to the Absorption Signal.

We propose to replace the traditional time-frequency domain filtering with feature domain filtering to realize an innovation of filtering algorithm. A feature domain transform filter (FDTF) is composed of the feature domain transform layer based on principal component analysis (PCA) algorithm, the feature domain information extractor based on deep learning and the time domain transform layer. It is established to filter out the noise with the same frequency and phase as the signal and is verified on methane gas.

BMP15 Modulates the H19/miR-26b/SMAD1 Axis Influences Yak Granulosa Cell Proliferation, Autophagy, and Apoptosis.

Bone morphogenetic protein 15 (BMP15) regulates the growth and development of follicles. In particular, the long non-coding RNA H19 plays an important role in mammalian reproduction. However, the function and regulatory mechanism of the interaction of BMP15 with H19 in yak granulosa cell (GC) proliferation, autophagy, and apoptosis are poorly understood.

[Accumulation Effects and Health Risks of Heavy Metals in Rice in Location-based Cadmium Anomaly Area in Liuzhou].

In order to explore the migration and transformation characteristics of soil heavy metals in rice in an area of ground source cadmium anomaly and to evaluate the safe planting of rice, a total of 91 pairs of soil and rice samples were collected from paddy fields in the typical area of Liuzhou city, Guangxi province, and the contents of heavy metals such as Cd, soil pH, and organic matter were tested. The results showed that:① Cd, Cu, Ni, and Zn in the paddy field exceeded the background values of 92.31%, 34.

A novel wavelength modulation spectroscopy gas sensing technique with an ultra-compressed wavelength scanning bandwidth.

In a wavelength modulation spectroscopy (WMS) gas sensing system, a scanning ramp combined with a high frequency sinusoidal signal is applied to drive the laser source. Generally, a wide wavelength scanning bandwidth realized by voltage scanning ramp is required to fully cover the target gas absorption profile. In this paper, a novel WMS-based strategy is proposed and verified in a CH detection system.

A Fourier-domain-based line shape recovery method used in direct absorption spectroscopy.

A novel absorption line shape recovery method with self-calibration function and ultra-easy implementation was introduced to direct absorption spectroscopy (DAS) in this study. The self-calibration function empowered the DAS system with the immunity to the laser power fluctuations. The ultra-easy implementation was achieved in that the DAS system was substantially simplified with a single-path DAS rather than the traditional dual-path differential optical absorption spectroscopy (DOAS), and the absorption profile can be directly recovered by an analog or digital filter instead of complex fitting algorithm or sophisticated balance detection circuits.

Recovery integral absorbance method in the full concentration range to eliminate the interference of background gas.

At present, gas sensors are extremely susceptible to interference from background gases in the field environment, which leads to greatly reduced accuracy. For this reason, we propose an improved method of recovering integral absorbance (IA) using Y component of first harmonic to achieve accurate prediction of the full range of concentration (not reaching absorption saturation). This approach can eliminate the interference of background gas at a low modulation depth (m < 0.

Fluorescence hyperspectral imaging system for analysis and visualization of oil sample composition and thickness.

In this paper, a compact fluorescence hyperspectral imaging system based on a prism-grating-prism (PGP) structure is designed. Its spectrometer spectral range is 400-1000 nm with a spectral resolution of 2.5 nm, and its weight is less than 1.