Inhibition effect of non-contact biocontrol bacteria and plant essential oil mixture on the generation of -nitrosamines in deli meat during storage.

To reduce the risk of -nitrosamines in deli meat products, this study formulated a novel non-contact -nitrosamines inhibiting preservative IV (NIP-IV) consisting of biocontrol bacteria and plant essential oils (EOs) ( SR-1 +  PP-2 +  CF-3+ cinnamon EO + grapefruit EO). Luncheon pork, spiced beef, and red sausage were taken as representatives of typical deli meat products and used to validate the effectiveness of NIP-IV in inhibiting -nitroso dimethylamine (NDMA) production. The results showed that NIP-IV restrain protein degradation and lipid oxidation in deli meat products and effectively control microbial activity.

Purification of soybean cupins and comparison of IgE binding with peanut allergens in a population of allergic subjects.

Identification, purification and characterization of allergens is crucial to the understanding of IgE-mediated disease. Immunologic and structural studies with purified allergens is essential for understanding relative immunogenicity and cross-reactivity. In this work, the complex soybean 7S vicilins (Gly m 5) with three subunits and 11S legumins (Gly m 6) with five subunits were purified and characterized along with purified peanut allergens (Ara h 1, 2, 3, and 6) by label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Evaluating the potential allergenicity of dietary proteins using model strong to non-allergenic proteins in germ-free mice.

Currently no validated animal model is predictive of human responses in ranking purified dietary proteins in the prevalence or potency of food allergy in humans. Since the gastrointestinal microbiota is thought to influence oral tolerance, we hypothesize that a germ-free mouse model will more accurately predict atopic human responses than conventional mice. Germ-free C3H/HeN mice were immunized with 60 μg Ara h 2, BLG, or LOX by three weekly intraperitoneal (IP) injections with alum adjuvant.

Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Genetic tool development in marine protists: emerging model organisms for experimental cell biology.

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa.

Conserved miRNAs and their targets identified in lettuce (Lactuca) by EST analysis.

MicroRNAs (miRNAs) are a newly identified class of endogenous, non-coding, short ( approximately 21nt) RNAs that play important roles in regulating gene expression at post-transcriptional level by targeting mRNA cleavage or translational inhibition in plants and animals. Though there are lots of differences between plant miRNAs and animal miRNAs, most of these tiny RNAs are highly conserved in each kingdom. Here, we show the conserved miRNAs in lettuce (Lactuca) identified using EST (expressed sequence tag) analysis.

Computational identification of microRNAs and their targets in wheat (Triticum aestivum L.).

microRNAs (miRNAs) are a class of endogenous, non-coding, short (approximately 21 nt) RNAs directly involved in regulating gene expression at the post-transcriptional level. Previous reports have noted that plant miRNAs in the plant kingdom are highly conserved, which provides the foundation for identification of conserved miRNAs in other plant species through homology alignment. Conserved miRNAs in wheat are identified using EST (Expressed Sequence Tags) and GSS analysis.