[Retracted] Fundamental research on the action mechanism of the 800 nm semiconductor laser on skin blackheads and coarse pores.

[This retracts the article DOI: 10.3892/etm.2016.

Reliability and reproducibility of CBCT assessment of mandibular changes before and after treatment for Class III growing patients - an easy and quick way for evaluation.

The objective of this study was to evaluate intraobserver reliability and inter-observer reproducibility of a 3-dimensional (3D) assessment method for mandibular changes of growing patients after orthodontic treatment for Class III malocclusion.Methods Cone-beam computed tomography (CBCT) scans were performed before and after orthodontic treatment for 27 patients. During the scan, the patient was positioned such that his/her mandibular plane was parallel to floor.

Icariin contributes to healing skull defects in rabbit model.

Objective: To investigate the efficacy of icariin for healing skull defects in rabbit models.

Methods: Thirty-six 3-month-old female New Zealand white rabbits, weighing 1.8-2.

Treatment of obstructive sleep apnoea-hypopnea syndrome by mandible advanced device reduced neuron apoptosis in frontal cortex of rabbits.

Objective: To investigate effects of mandible advanced device (MAD) therapy for obstructive sleep apnoea-hypopnea syndrome (OSAHS) on the neuron apoptosis and acetylcholine esterase activity in frontal cortex.

Materials And Methods: Thirty male New Zealand white rabbits were randomly divided into three groups (n = 10 in each group): group OSAHS, group MAD, and control group. Hydrophilic polyacrylamide gel was injected into soft palate of the animals to induce OSAHS in group OSAHS and group MAD.

Fundamental research on the action mechanism of the 800 nm semiconductor laser on skin blackheads and coarse pores.

The aim of the study was to determine the mechanism of action of the 800 nm semiconductor laser on skin blackheads and coarse pores. A total of 24 healthy purebred short-haired male guinea pigs, weighing 350-400 g, were selected and smeared with 0.5 ml coal tar suspension evenly by injector once daily.

The effects of increasing occlusal vertical dimension on the deep masseter of rat at different ages.

Objective: To investigate the influence of increasing the occlusal vertical dimension (iOVD) on the fibre-type distribution and ultrastructure of deep masseter of rat at different ages.

Design: A total of forty-eight male Wistar rats were divided into two groups according to age: 'teenage' group (n=24, 1.5 months) and 'young adult' group (n=24, 8 months).

Effects of a mandibular advancement device on genioglossus in obstructive sleep apnoea hypopnea syndrome.

Objective: To investigate effects of mandibular advancement device (MAD) therapy for obstructive sleep apnoea hypopnea syndrome (OSAHS) on the genioglossus contractile properties and fibre-type distribution.

Materials And Methods: Thirty 6-month old male New Zealand white rabbits were randomised into three groups: OSAHS, MAD, and controls. Rabbits in Group OSAHS and Group MAD were established as OSAHS models by injection, at a dose of 2 ml hydrophilic polyacrylamide gel, via the submucous muscular layer of soft palate.

[Establishment of the craniofacial three-dimensional finite element models with the sutures defined alone].

Objective: To develop a child craniofacial three-dimensional (3D) finite element model (FEM) with sutures defined alone.

Methods: The CT data for this study was developed from sequential computed tomography scan images taken at 0.625 mm intervals of an 8 years children skull.

[The effect of mini-implant lengths on stress distributions in peri-implant surface].

Objective: To observe the effect of mini-implant lengths on stress distribution in peri-implant surface.

Methods: The 3D finite element analysis mandible and mini-implant models with diameter of 1.6 mm, lengths of 6, 8, 10 and 12 mm were established.

[Therapeutic effect of emodin in experimental periodonititis rats].

Aim: Through observing the change of Nitric Oxide (NO) in the peripheral blood and gingival tissue, to explore the mechanism of Emodin (EMD) in the therapy of periodontal disease.

Methods: SD rats were randomly divided into four groups of thirty (group N, group P, group PL and the group PH). The periodontitis model was made and EMD was administered at PL and PH.

[Effects of traditional Chinese medicine on the differentiation of mesenchymal stem cells to osteoblasts].

Objective: To investigate the effects of traditional Chinese medicine on the differentiation of mesenchymal stem cells (MSC) to osteoblasts.

Methods: Six male Beagle dogs weighed 10-15 kg each were divided into three groups, group A: medicine serum group, group B: non-medicine serum group and group C: bovine serum group. The serum of group A was obtained from the femoral artery of 2 Beagle dogs drinking equivalent dose of traditional Chinese medicine according to body surface area for 7 continuous days.

[Relationship between proteoglycans and proliferation of human salivary adencid cystic carcinoma].

Objective: To investigate the effect of down-regulated proteoglycans on the proliferation of human salivary adenoid cystic carcinoma (SACC).

Methods: The short hairpin RNA (shRNA) plasmid silencing human xylosyltransferase-I (XT-I) gene was constructed and named shRNA-WJ3. Adenoid cystic carcinoma cells with high metastatic tendency (ACC-M) were transfected by shRNA-WJ3.

[Effect of maxillary protraction with or without rapid palatal expansion in treating early skeletal Class III malocclusion].

Objective: To evaluate the effectiveness of treatment with maxillary protraction with or without rapid palatal expansion (RPE) for skeletal Class III malocclusion in mixed dentition.

Methods: A total of 31 children with Class III malocclusion in mixed dentition were selected, and 15 (group A) received maxillary protraction treatment with RPE, the other 16 (group B) received maxillary protraction without RPE. Cephalometric films were taken before and after treatment, and traditional and Pancherz analysis were used.

[Rapid canine distalization through distraction of the periodontal ligament after reducing interseptal bone resistance].

Objective: To investigate the effect of rapid canine distalization through distraction of the periodontal ligament after reducing interseptal bone resistance.

Methods: Twenty canines in 11 patients who needed first premolar extractions were involved. A tooth-borne, custom-made distractor was bonded right after the first premolar extraction and the interseptal bone resistance reduction.

[Effect of shRNA-mediated silence of H-ras gene on proliferation of human SACC-M cells].

Objective: To examine the effects of H-ras gene silence on cell cycle, proliferation and apoptosis of salivary adenoid cystic carcinoma -M (SACC-M) cell lines.

Methods: The plasmid H-ras-shRNA, containing the sequence of shRNA targeting H-ras, and HK-shRNA (without interfering effect) were constructed and transfected into SACC-M cells. The cell line with shRNA plasmid stable expression was isolated by G418.

[Preparation and identification of anti-human polyclonal antibody against xylosyltransferase-I].

Aim: To prepare the rabbit polyclonal antibody against human xylosyltransferase-I (XT-I) protein and to identify its specificity.

Methods: The predominant epitope of XT-I gene was predicted by the DNAssist software. The DNA fragment of this epitope region was synthesized by PCR and cloned into the pGEX-4T-2 vector.

[Study on effect of snore guard to upper airway structure of normal occlusion people by magnetic resonance imaging].

Objective: To investigate the imagery changes of the upper airway and the surrounding soft tissues of local adults with non-apnea who used snore guard and to provide experimental data for the clinical diagnosis and treatment of obstructive sleep apnea syndrome (OSAS).

Methods: Thirty students with non-apnea from Hebei medical university were chosen, and magnetic resonance imaging (MRI) was used to measure the changes of the upper airway and the surrounding soft tissues after snore guards were used. SPSS 105 software was used to analyze statistically.

[An experimental study on porcine fibroblasts transfected by dental matrix protein-1 gene].

Objective: To evaluate the expression of dental matrix protein-l (DMP1) in porcine oral mucosa fibroblasts (POMF) transfected by DMP1 and the influences of the transfection.

Methods: The full length of porcine DMP1 cDNA was linked into an eukaryotic expression vector pEGFP-C1. POMF and mesenchymal stem cells (MSC) were transfected with the pEGFP-DMP1.

[wt-p53 gene repair for p53 mutations of salivary pleomorphic adenoma cells].

Objective: To analyze the original mutated codon of p53 gene of salivary pleomorphic adenoma (SPA) and to evaluate the repair effects of wt-p53 on SPA cells.

Methods: Four cases of SPA were obtained from clinical fresh samples and SPA cells were separated and cultured, and then the cells were transduced by Ad-wt-p53. The cells and the corresponding tumor tissue DNA were extracted, PCR and single strand conformational polymorphism (SSCP) and DNA sequencing analysis were performed.

[Expression of p53 gene with DNA polbeta and CMV promoter in salivary adenoid cystic carcinoma cells].

Objective: To evaluate the activity of DNA polbeta promoter on p53 gene in salivary adenoid cystic carcinoma (SACC) cells.

Methods: The luciferase activity was examined and used to evaluate the activity of DNA polbeta promoter on SACC-83 cells. Eukaryotic expression plasmids of p53 gene were constructed and stably transfected into SACC-83 cells.

[Study of p16INK4/CDKN2 gene homozygous deletions and point mutation in squamous cell carcinoma of buccal mucosa].

Objective: To explore the correlation between homozygous deletions and mutation of p16 gene and the carcinogenesis and progression of squamous cell carcinoma of buccal mucosa.

Methods: Thirty buccal cancers, 10 leukoplakias and 8 buccal mucosas were involved. DNA was extracted from the tissues.

[The relationship between methylation of p16 INK 4/CDKN2 gene in promoter region and progress of squamous cell carcinoma of buccal mucosa].

Objective: To determine the correlation between methylation of p16 gene in promoter region and the carcinogenesis and progression of squamous cell carcinoma (SCC) of buccal mucosa.

Methods: Methylation of pl6 gene in SCC and leukoplakia of buccal mucosa was investigated by MSP and pl6 protein was analyzed by Western blot.

Results: The methylation of p16 gene was found in 15 of 30 cases SCC and 1 of 10 cases of leukoplakia of buccal mucosa (P < 0.

[Inhibitory effect of p53 gene on telomerase activity and proliferative activity in salivary adenoid cystic carcinoma cells].

Objective: To evaluate the inhibitory effect of p53 gene on salivary adenoid cystic carcinoma (SACC) cells.

Methods: Adenoviral vector pDeltaE1-p53 was constructed and transfected into SACC-83 cells. The enhanced p53 expression was measured by reverse transcription polymerase chain reaction (RT-PCR), and the effects of transfected p53 on SACC-83 cells were analyzed by TRAP-PCR-ELISA, luciferase reporter, flow cytometry (FCM), soft agar assay and tumorigenicity test.

[Effect of early treatment with the Twin-block appliance].

Objective: To evaluate the effectiveness of early treatment with the Twin-block appliance for the developing Class II division 1 malocclusion.

Methods: A Total of 30 children with Class II division 1 malocclusion, 18 (8 male, 10 female) out of which received treatment with the Twin-block appliance, the other 12 cases (6 male, 6 female) without treatment served as control group. Cephalometric data were collected at the start and the end of the study and statistical analysis were applied.