Association between copy number variation of gene and growth traits in Chinese cattle.

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 () belongs to the serine protease inhibitor family A subtype, and contains 8 genes from to . Although the regulatory effects of these 8 genes have been revealed one by one in recent years, the related effects of gene on cattle growth is still unclear. This study used quantitative Real time PCR (qPCR) to detect the type of copy number variation (CNV) of gene in a total of 542 Chinese cattle, and expression of gene in different tissues of Qinchuan cattles (adult) on mRNA level.

Determination of genetic effects of on important growth traits in beef cattle.

Serine protease inhibitor protein 3 (serpin peptidase inhibitor, clade A, member 3, ) is a member of the serpin superfamily, probably related to the yield and quality of muscle. This study focuses on the relationship between gene polymorphism and growth traits in beef cattle. The study first uses sequencing pooled DNA samples (Pool-Seq), PCR-RFLP and Tetra-primer ARMS-PCR techniques to determine the genetic polymorphisms of SERPINA3 in 765 beef cattle.

H19 lncRNA regulates keratinocyte differentiation by targeting miR-130b-3p.

Aberrant differentiation of keratinocytes has been demonstrated to be associated with a number of skin diseases. A growing number of studies have showed that long noncoding RNAs (lncRNAs) have an important part in gene regulation, however, the role of lncRNAs in keratinocyte differentiation remains to be largely unknown. In the present study, we demonstrated that lncRNA-H19 act as an endogenous 'sponge', which binds directly to miR-130b-3p and therefore inhibits its activity on Dsg1.

A large indel mutation of the bovine ADD1/SREBP1c gene and its effects on growth traits in some native cattle breeds from China.

Adipocyte determination and differentiation-dependent factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP1c) is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. The objectives of the present study were to focus on insertion-deletion polymorphism (indel) in the bovine ADD1/SREBP1c gene, and analyzing its effect on growth traits in a sample of 1035 cattle belonging to four Chinese cattle breeds. PCR-SSCP, DNA sequencing and agarose electrophoresis methods were used.

[Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian red cattle].

The polymorphisms of DGAT2 gene in 92 Jiaxian red cattle were analyzed using PCR-RFLP method. Results showed that the PCR products digested with TaqI demonstrated polymorphisms, and showed two kinds of genotype: AA and AB. This polymorphic locus of DGAT2 gene was at Hardy-Weinberg equilibrium (P>0.