Regional Injection of CAR-T Cells for the Treatment of Refractory and Recurrent Diffuse Large B Cell Lymphoma: A Case Report.

Background: Lymphoma is a common hematological malignancy with many subtypes and considerable heterogeneity. Traditional treatments include chemotherapy, radiotherapy, and surgery. Patients with relapsed, refractory or advanced stage lymphoma have a dismal prognosis.

Expression of key ion channels in the rat cardiac conduction system by laser capture microdissection and quantitative real-time PCR.

The objective of this study was to investigate the molecular basis of the inferior nodal extension (INE) in the atrioventricular junctional area that accounts for arrhythmias. The INE was separated from the adult rat heart by laser capture microdissection. The mRNA expression of ion channels was detected by quantitative real-time PCR.

[Electrophysiologic characteristics of Ito in myocytes from cardiac Koch triangle].

Objective: To explore the electrophysiologic characteristics of Ito in myocytes from cardiac Koch triangle.

Methods: Patch clamp technique was employed to investigate the I-V and D-V relation, steady-state activation and inactivation kinetics of Ito from myocytes in Koch triangle of rabbit hearts.

Results: (1) The maximum peak current (pA) and peak current density (pA/pF) at +20 mV in PC (pacing cell), TC (transitonal cell) alpha,beta, AC (atrial cell) and PL (purkinje-like cell) cells were different from each other (P < 0.

Sodium current kinetics of transitional myocytes in Koch triangle of rabbit hearts.

Background: Few studies have explored the inward sodium current (INa) kinetics of transitional cardiomyocytes. This study aimed to explore the kinetics of transitional cardiomyocytes types alpha and beta.

Methods: The whole-cell patch clamp technique was used to study the rapid INa of isolated transitional cardiomyocytes in the Koch triangle of rabbit hearts.

[Quantitative connexin mRNA detection in posterior nodal extension of adult rat heart].

Objective: To quantitatively detect the expression of connexins (Cx) mRNA in the posterior nodal extension (PNE) of adult rat heart and understand the relationship between Cx expression and atrial ventricular nodal reentrant tachycardia (AVNRT).

Methods: PNE was separated from adult rat heart by means of laser microdissection (LCM), and the cells were also isolated from the atrioventricular node (AVN), sinoatrial node (SAN), Purkinje fiber (PF), right atrium (RA) and right ventricle (RV), to serve as the controls. The Cx mRNA level was detected in these cells with quantitative real-time PCR (QRT-PCR).

Morphological and electrophysiological properties of single myocardial cells from Koch triangle of rabbit heart.

Background: The morphological and electrophysiological characteristics of cardiac cells in Koch triangle are still disputed. We studied the appearance and electrical properties of these diverse myocytes to elucidate their complex electrophysiological phenomena.

Methods: Experiments were conducted using cooled charge coupling device (CCD) system and whole cell, patch clamp technique to determine the morphology, action potential and sodium current density of single viable myocytes enzymatically isolated from the Koch triangle of rabbit hearts.

[An application of laser capture microdissection to isolate the pure atrial cells].

Objective: To use the laser capture microdissection (LCM) to isolate the pure atrial cells from heart. To extract tiny amount RNA and amplify the genes of beta-actin and GAPDH.

Methods: First, the prepared sample RNA was extracted and verified.

[Expression of L-type calcium channel alpha1C subunit in adult rat heart].

Objective: To investigate the expression and distribution of L-type calcium channel alpha1C subunits in adult rat heart.

Methods: HE staining was applied on the frozen sections of adult rat heart to identify the sinoatrial node (SAN), atrioventricular node (AVN), and posterior nodal extension (PNE). The protein expression of L-type calcium channel alpha1C in adult rat heart and its cellular localization were examined by Western blotting and immunohistochemistry, respectively.