Genetic Susceptibility to Norovirus GII.4 Sydney Strain Infections in Taiwanese Children.

Background: A comprehensive evaluation of associations between the susceptibility to norovirus infections and histo-blood group antigens is not available in the Taiwanese population, in which the nonsecretor phenotype is absent.

Methods: A 1:1 matched case-control study was conducted in northern Taiwan from February 2013 to December 2014 when an epidemic of norovirus infection occurred. Cases were children <18 years old who were hospitalized because of diarrhea and were found to have laboratory-confirmed norovirus infections.

Annexin A2, up-regulated by IL-6, promotes the ossification of ligament fibroblasts from ankylosing spondylitis patients.

Background: Annexin A2, a calcium-dependent phospholipid binding protein, is involved in osteogenesis. The objective of the present study was to explore the expression of Annexin A2 in spinal ligament tissues (LT) of ankylosing spondylitis (AS) patients and determine its pathological functions.

Methods: mRNA and protein expression of Annexin A2 was detected by real-time PCR and Western blotting, respectively.

[Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans].

Purpose: To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro.

Methods: By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of siRNA for fluoride resistant Streptococcus mutans was screened. In different values of pH of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the pH and OD600.

[Serological survys on anti-H1N1 IgG of blood donors in Dongguan].

Objective: To access the sustained immune effect in influenza A H1N1 vaccine vaccinated-blood donors as well as the level of anti-H1N1 IgG in unvaccinated-blood donors in order to provide reference for preventing and treating influenza A H1N1.

Methods: Anti-H1N1 IgG was detected in 1166 vaccinated-blood donors as well as 1265 unvaccinated-blood donors by ELISA method in Dongguan from January 2010 to June 2010.

Results: The mean positive rate and high-titer rate of anti-H1N1 IgG were 78.

[Source and air-sea fluxes of heavy metals in the atmospheric particles of East China Sea].

From 2006 to 2007, four surveys for marine atmosphere in East China Sea were carried out included different seasons (spring, summer, autumn and winter). Based on the survey data of heavy metals in marine atmospheric particles, analyzed the source of heavy metals by the calculation of Enrichment Factors, calculated the air-sea fluxes via dry deposition. The results showed, comparing with the crustal and seawater, the heavy metals including Cu, Pb, Cd and Zn were highly concentrated, mainly from the pollutants of human activities.

[Determination of electron density in atmospheric pressure radio frequency dielectric barrier discharges by Stark broadening].

The use of high frequency power to generate plasma at atmospheric pressure is a relatively new development. An apparatus of atmospheric pressure radio frequency dielectric barrier discharge was constructed. Plasma emission based measurement of electron density in discharge columns from Stark broadening Ar is discribed.

Transplantation of human umbilical cord blood mesenchymal stem cells improves survival rates in a rat model of acute hepatic necrosis.

Introduction: Stem cell-based therapies are emerging as important and promising methods in the treatment of end-stage liver disease. This study is aimed to evaluate the effects of human umbilical cord blood mesenchymal stem cell (HUCBMSC) transplantation in acute hepatic necrosis (AHN).

Methods: Green fluorescent protein (GFP)-labeled HUCBMSCs were injected into the liver of rats in which AHN was induced by carbon tetrachloride, and the migration of these cells in liver slices was evaluated from 48 hours to 4 weeks post-transplantation.

The manual Polybrene test has limited sensitivities for detecting the Kidd blood group system.

Background And Objectives: The Kidd system antibodies, if undetected, can cause immediate or delayed hemolytic transfusion reactions as well as hemolytic disease of the newborn. There have been anecdotal reports about the inefficiency of the manual Polybrene test in detecting these antibodies. Here, we sought to determine the sensitivity of the manual Polybrene test in detecting anti-Jk(a) and anti-Jk(b) antibodies and Jk(a) and Jk(b) antigens.

[Study about seroconversion of HBV NAT screening-positive crowd from blood donors].

Objective: To investigate the characteristics of seroconversion of HBV NAT screening-positive crowd from blood donors in Dongguan city and provide reference for the safety of blood transfusion and disease prevention.

Methods: With retrospective survey, Nucleic acid testing (NAT) was used to analyze 28800 HBsAg-negative samples by ELISA from blood donors in Dongguan city from August, 2006 to August, 2007 with Roche Cobas AmpliScreen systems; and follow-up research including NAT for HBV-DNA, ELISA for HBsAg and multiple factors analysis for HBV infection was carried out on HBV NAT screening-positive crowd.

Results: 10 positive pooling were screened from 28800 samples; after further detection, 2 of these positive pooling were HBV-DNA negative and 8 HBV-DNA positive samples were found.

[Analysis of DC-SIGN and DC-SIGNR genetic polymorphism in Chinese Han population].

Objective: To understand the genetic polymorphism of DC-SIGN's and DC-SIGNR's neck regions in normal Chinese Han population, and to obtain the genetic data of the two loci in Chinese Han population.

Methods: The genotypes and alleles of repeat sequences of DC-SIGN and DC-SIGNR neck region were typed by PCR, agarose gel electrophoresis and sequencing. Polymorphism information content (PIC) of DC-SIGNR was calculated.

[Comparison of homemade and imported HbsAg ELISA kits on screening blood samples].

Background: To evaluate homemade and imported HbsAg ELISA kits on screening blood donors.

Methods: Samples for evaluation included 120 HbsAg serum plates for the golden criteria and 400 sets of serum from blood donors in Dongguan. The samples underwent blind screening with homemade and imported ELISA kits respectively.