Design and synthesis a mitochondria-targeted dihydronicotinamide as radioprotector.

Radiation-induced damage to the mitochondrial macromolecules and electron transfer chain (ETC), causing the generation of primary and secondary reactive oxygen (ROS) species. The continuous ROS production after radiation will trigger cell oxidative stress and ROS-mediated nucleus apoptosis and autophagy signaling pathways. Scavenging radiation-induced ROS effectively can help mitochondria to maintain their physiological function and relief cells from oxidative stress.

[Effects of different cultivation modes on the leaf photosynthetic characteristics and yield of summer-sowing peanut].

Taking the Arachis hypogaea cv. 'Qinghua 7' as test material, a field experiment was conducted to study the effects of different cultivation modes on the leaf photosynthetic characteristics and yield of summer-sowing peanut after wheat harvest. As compared with conventional cultivation mode, high-yield protective cultivation mode promoted the leaf growth, significantly improved the leaf area index (LAI), and maintained a longer time of high LAI and chlorophyll content.

[Production and releasing sites of superoxide anion in phytopathogenic bacteria].

Many phytopathogenic bacteria were founded to release the superoxide anion (O2-) by themselves from different sites and the production of O2- was related to the pathogenicity of strains. The presences of O2- in cytomembrane, cell wall and filtrate were confirmed by chemical method and electron spin resonance (ESR). The results show that filtrate may be the primary site of O2- production due to its higher O2- generating activity either in the presence or the absence of DDC.

Experimental study on the new significant function domains of KCHIP1 protein.

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Theoretically KCHIP1 has several calcium binding domains and two myristoylation sites. In this study, we demonstrated that the calcium binding domains and myristoylation sites were functional.

[O2-* burst of tobacco leaves triggered by Erwinia carotovora subsp. carotovora inoculation].

O(-.)(2) production based on chemical method by Ecc-treated intact tobacco leaves was observed. It showed a long-lasting one-phase course beginning 1 h and ending 14 h after Erwinia carotovora subsp.

[Discovery of a new splicing type of KCHIP1 gene].

The present study aimed to find the mutations of KCHIP1 gene in breast cancer. KCHIP1 cDNA samples from 12 specimens of breast cancer and 12 specimens of normal mammary tissues were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and directly sequenced to detect mutation. No mutation of KCHIP1 gene was found in these samples; while a new splicing type of KCHIP1 gene was found, which has an insert (162 bp) between exon 1 and exon 2 in KCHIP1 gene (AY780424).

[Expression of Fas, FasL, and NF-kappa B in the process of osteoclast-like cell apoptosis effected by sodium fluoride].

Objective: To detect the changes in the expression of apoptosis signals: Fas, FasL and NF-kappa B in the process of osteoclast-like cell (OLC) apoptosis effected by sodium fluoride.

Methods: After co-culture of osteoclast-like cells with 0, 5, 10 and 15 mg/L sodium fluoride, Fas, FasL and NF-kappa B antibody expressions were detected by immune-histochemistry.

Results: The expression of Fas and FasL increased with the concentration of the sodium fluoride, however the expression of NF-kappa B decreased with the concentration of sodium fluoride.