Publications by authors named "Fu-Jia Liu"
Characterization of a novel tetravalent botulism antitoxin based on receptor-binding domain of BoNTs.
Appl Microbiol Biotechnol
May 2023
Article Synopsis
- Botulinum neurotoxins (BoNTs) cause botulism in humans, which can be treated effectively with antitoxins; researchers developed a new antitoxin using recombinant C terminal heavy chain domains from BoNTs.* -
- The initial antitoxins created (M-BATs) targeted individual BoNT serotypes (A, B, E, F) but lacked cross-protection, leading to the need for a more comprehensive tetravalent antitoxin (T-BAT) capable of neutralizing all four at once.* -
- The T-BAT demonstrated strong effectiveness in animal models, binding efficiently to the receptor-binding domain (RBD) of the toxins, suggesting this strategy
Botulinum neurotoxins (BoNTs) are the most toxic known proteins. Naturally occurring botulism in humans is caused by botulinum serotypes A, B, E, and F. Vaccination is an effective strategy to prevent botulism.
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- This study explores the timing and methods for assessing measurable residual disease (MRD) in acute myeloid leukemia (AML), suggesting that MRD assessment after two cycles of consolidation treatment is more effective than after induction.
- The results show that multiparameter flow cytometry (MFC) MRD provides significant prognostic value for relapse-free and overall survival in AML patients, especially when analyzing specific gene mutations.
- Combining MFC MRD with molecular MRD techniques can enhance risk stratification, though the choice of method should depend on the patient's genetic characteristics related to AML.
Article Synopsis
- Acute myeloid leukaemia (AML) presents a complex prognosis due to various patient and disease factors, despite new treatment advancements.
- A study identified several key prognostic factors (like age and genetic mutations) that can help predict overall survival, leading to a predictive model for individual risk assessment.
- This model shows strong performance in differentiating survival outcomes for young and elderly patients and could assist in treatment decisions, particularly for stem cell transplant eligibility.
Article Synopsis
- * Tests on mice showed that the THc-based vaccine provided complete protection against tetanus, outperforming the current full-length TT vaccine in generating stronger immune responses.
- * The THc protein also interacts with a specific ganglioside (GT1b), and the antibodies generated from the THc vaccine can block this interaction, indicating that the recombinant THc could serve as an effective subunit vaccine against tetanus.
Botulinum neurotoxins (BoNTs) are highly toxic proteins that mediate their effects by binding to neuronal receptors and block the neutralizing ability of therapeutic antibodies. Vaccination is currently the most effective strategy to prevent botulism. In this study, a series of recombinant functional domain antigens of BoNT/A were prepared and identified, and their immunoprotective efficacies were explored and compared.
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- A study compared clinical and genetic characteristics among elderly AML (e-AML), secondary AML (s-AML), and young de novo AML patients, finding distinct mutation patterns and lower blood cell counts in e-AML and s-AML groups.
- e-AML and s-AML patients had lower rates of complete remission and shorter survival times compared to younger patients, indicating a need for different treatment strategies.
- Intensive therapy may enhance survival for e/s-AML patients without increasing early death risks, and hematopoietic stem cell transplantation is recommended for treating fit s-AML patients.
Botulinum neurotoxins (BoNTs) are among the most toxic proteins. Vaccination is an effective strategy to prevent botulism. To generate a vaccine suitable for human use, a recombinant non-His-tagged isoform of the Hc domain of botulinum neurotoxin serotype E (rEHc) was expressed in and purified by sequential chromatography.
View Article and Find Full Text PDFHypoxia inducible factor-1α facilitates cellular adaptation to hypoxic conditions. Hence its tight regulation is crucial in hypoxia related diseases such as cerebral ischemia. Changes in hypoxia inducible factor-1α expression upon cerebral ischemia influence the expression of its downstream genes which eventually determines the extent of cellular damage.
View Article and Find Full Text PDFMicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge.
View Article and Find Full Text PDFBackground: Hyperbaric oxygen preconditioning (HBO) is a new method of ischemia preconditioning. In this study, we examined its effects on skin flap survival and the mechanisms involved.
Methods: Thirty-six rats were divided into three groups: HBO preconditioning, control, and sham groups.
To date, miRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were harvested at different reperfusion time points ranging from 0-168 hrs after middle cerebral artery occlusion using homologous emboli.
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