Active immunization with recombinant GnRH6-CRM197 inhibits reproductive function of male rats.

Gonadotropin-releasing hormone (GnRH) vaccines have been successfully used for the inhibition of gonadal development and function, but current GnRH-based vaccines often present variability in the response. Cross-reactive material 197 (CRM197) has been used as carrier molecules to enhance an immune response to associated antigens. So, the synthetic mammalian tandem-repeated GnRH hexamer (GnRH6) gene was integrated into the expression plasmid pET-21a.

Identification of lncRNAs by RNA Sequencing Analysis During Pre-Implantation Developmental Transformation in the Goat.

Pre-implantation development is a dynamic, complex and precisely regulated process that is critical for mammalian development. There is currently no description of the role of the long noncoding RNAs (lncRNAs) during the pre-implantation stages in the goat. The transcriptomes of oocytes (n = 3) and pre-implantation stages (n=19) at seven developmental stages in the goat were analyzed by RNA sequencing (RNA-Seq).

miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat.

This study found that miR-27 is expressed in muscle and regulates muscle proliferation and differentiation. We explored the function and regulatory mechanism of miR-27b in goat muscle proliferation and differentiation. Compared with the Boer goat, higher expression of miR-27b was observed in all of the collected muscle tissues of Anhuai goat, excluding the kidney, whereas the opposite expression pattern was observed for Pax3, which showed lower expression in Anhuai goat.

[Functional genomics studies of Salvia miltiorrhiza II--gene expression profiling of different stage of hairy root].

Objective: Studying the gene expression profiling of different stage hairy root of Salvia miltiorrhiza, in order to find functional genes.

Method: The contents of second metabolites were determined by HPLC and gene expression profiling was detected by cDNA microarray. cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine's linear RNA amplification method and subsequent enzymatic reaction.

[Functional genomics studies of Salvia miltiorrhiza I. Establish cDNA microarray of S. miltiorrhiza].

Objective: Establishing cDNA microarray, in order to study functional genomics of Salvia miltiorrhiza.

Method: Total RNA samples were prepared from S. miltiorrhiza roots using a modified CTAB method.