Publications by authors named "Fu-Chuan Li"
Alginate lyases, which are important in both basic and applied sciences, fall into ten polysaccharide lyase (PL) families. PL36 is a newly established family that includes 39 bacterial sequences and one eukaryotic sequence. Till now, the structures or catalytic mechanisms of PL36 alginate lyases have yet to be revealed.
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- Agarase is an enzyme that breaks down agarose into oligosaccharides, with its glycoside hydrolase (GH) module being crucial for this activity.
- The agaG4 gene from the Flammeovirga strain MY04 encodes a unique 503-amino acid protein, AgaG4, which has an additional peptide in its GH module compared to other known agarases.
- Modifying AgaG4 by removing the extra peptide showed that it affected the enzyme's ability to degrade agarose, optimal temperature, and substrate cleavage pattern, highlighting the importance of a specific residue, tyrosine 276, in its activity.