Publications by authors named "Frolova G"

The high incidence of epithelial malignancies in HIV-1 infected individuals is associated with co-infection with oncogenic viruses, such as high-risk human papillomaviruses (HR HPVs), mostly HPV16. The molecular mechanisms underlying the HIV-1-associated increase in epithelial malignancies are not fully understood. A collaboration between HIV-1 and HR HPVs in the malignant transformation of epithelial cells has long been anticipated.

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A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus.

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A Gram-stain-negative, aerobic, yellow-pigmented, heterotrophic, sulfur-oxidizing, non-motile, rod-shaped bacterium, designated KMM 3898(T), was isolated from an internal tissue of the sand snail Umbonium costatum, collected from the shallow sediments of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KMM 3898(T) formed a distinct phylogenetic lineage within the class Gammaproteobacteria and was most closely related to Leucothrix mucor DSM 2157(T) (89.2 % 16S rRNA gene sequence similarity) and members of the genus Thiothrix (86.

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Two Gram-negative, strictly aerobic, non-motile bacteria designated strains KMM 3851(T) and KMM 3842(T) were respectively isolated from a marine snail specimen (Umbonium costatum) and from surrounding sediments collected simultaneously from the shore of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains KMM 3851(T) and KMM 3842(T) were affiliated with the Roseobacter lineage of the class Alphaproteobacteria as a separate phylogenetic line adjacent to the members of the genus Thalassobacter. These novel isolates shared 98.

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Three Gram-negative, aerobic, halophilic, non-motile, yellowish-pigmented bacteria, designated KMM 3896, KMM 3897 and KMM 3899(T), were isolated from coastal seawater and sediment samples of the Sea of Japan, Russia. The isolates were shown to belong to the same species on the basis of 16S rRNA gene sequence similarities (99.6-99.

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A Gram-negative, aerobic, yellow-pigmented, rod-shaped, non-motile bacterium, strain KMM 3895T, was isolated from a marine sandy sample collected offshore from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KMM 3895T in the class Gammaproteobacteria, forming a separate branch, sharing 89.5 % sequence similarity with Nitrincola lacisaponensis 4CAT and 88-87% similarity with the other members of the cluster, including members of Kangiella, Spongiibacter, Alcanivorax and Microbulbifer.

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A Gram-negative, aerobic, greyish-yellowish-pigmented, stenohaline, rod-shaped, non-motile bacterium, strain KMM 3900(T), was isolated from a coastal seawater sample collected from the Sea of Japan. Based on phylogenetic analysis, strain KMM 3900(T) was positioned within the Gammaproteobacteria on a separate branch adjacent to members of the genera Reinekea and Kangiella, sharing less than 88 % 16S rRNA gene sequence similarity with all recognized species of the Gammaproteobacteria. The major isoprenoid quinone was Q-8.

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Article Synopsis
  • Two new bacterial strains, KMM 3891(T) and KMM 3892, were discovered in the internal tissues of the marine mollusc Umbonium costatum from the Sea of Japan.
  • The bacteria are categorized as Gram-negative, non-motile, psychrotolerant rods with specific lipid and fatty acid compositions, and unable to degrade complex polysaccharides.
  • Phylogenetic analysis shows these strains belong to a new genus and species called Umboniibacter marinipuniceus, distinct from related bacteria, with KMM 3891(T) designated as the type strain.
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The phenotypic and phylogenetic characteristics of an aerobic, Gram-negative, motile, non-pigmented Alteromonas-like bacterium (designated strain KMM 3894(T)), isolated from a sandy sediment sample collected offshore of the Sea of Japan, were investigated. Comparative 16S rRNA gene sequence analysis revealed that strain KMM 3894(T) belonged to the genus Aestuariibacter and was most closely related to Aestuariibacter halophilus JC2043(T) (95.5 % sequence similarity).

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A Marinomonas-like bacterium, strain KMM 3893(T), was isolated from a marine sandy sediment collected close to shore from the Sea of Japan and subjected to a phenotypic and phylogenetic study. Comparative 16S rRNA gene sequence analysis confirmed the novel strain's assignment to the genus Marinomonas. Strain KMM 3893(T) constituted a separate phyletic line in the genus Marinomonas, sharing <97 % sequence similarity with respect to other recognized Marinomonas species.

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Article Synopsis
  • Two novel strains of Psychrobacter bacteria, KC 40(T) and KC 65, were isolated from a marine crustacean in the Sea of Japan and identified through a comprehensive analysis.
  • These strains showed the ability to produce unique black-brown pigments, a distinct feature of the Psychrobacter genus, and formed a separate cluster phylogenetically from known species via gene sequencing.
  • Based on DNA-DNA hybridization and various physiological and biochemical traits, KC 40(T) and KC 65 were classified as a new species, named Psychrobacter fulvigenes sp. nov., with KC 40(T) designated as the type strain.
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  • A new bacterial strain, R60(T), was isolated from marine sediment in the Sea of Japan and is identified as a yellow-pigmented, aerobic, Gram-negative bacterium.
  • Genetic analysis indicates that R60(T) is part of the genus Winogradskyella, but with less than 97% similarity to already known species.
  • The strain has distinct fatty acids and phenotypic traits that support its classification as a new species, proposed to be named Winogradskyella arenosi sp. nov.
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  • A new bacteria strain, KC7(T), was isolated from a marine crustacean in the Sea of Japan and identified as part of the Sphingomonas genus through genetic analysis.
  • Strain KC7(T) shared 96.1% genetic similarity with existing Sphingomonas species, particularly S. trueperi, indicating its close relation but also its uniqueness as a separate lineage.
  • This strain has specific lipid and fatty acid characteristics typical of Sphingomonas, leading researchers to classify it as a new species named Sphingomonas japonica sp. nov.
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An aerobic, Gram-negative, non-pigmented, non-motile bacterium, KMM 3858(T), was isolated from a sea-ice sample collected from Peter the Great Bay of the Sea of Japan, Russia, and subjected to a phenotypic and phylogenetic study. Comparative analyses based on the 16S rRNA and recA gene sequences placed strain KMM 3858(T) within the genus Pseudochrobactrum. The major chemotaxonomic characteristics were found to be the presence of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminolipid and phosphatidylcholine, major fatty acids C(18 : 1)omega7c and C(19 : 0) cyclo, and ubiquinone Q-10, confirming the affiliation of strain KMM 3858(T) to the genus Pseudochrobactrum.

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An aerobic, Gram-negative, motile, non-pigmented bacterium, strain KMM 3042(T), isolated from a deep-sea brittle star in the Fiji Sea, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed marine Mn(II)-oxidizing isolate S185-2B as the closest neighbour of strain KMM 3042(T) (99.9 % sequence similarity).

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Recently, several blind cepstral deconvolution methods for medical ultrasound images were compared experimentally. The results indicated that the generalized cepstrum or the complex cepstrum with phase unwrapping give the blind homomorphic deconvolution algorithms with the best performance. However, the frequency domain phase unwrapping for pulse estimation, which is an essential part of both methods, is sensitive to the sensor noise when the values of the spectrum are small due to the randomness of the tissue response.

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An aerobic, Gram-negative bacterium, strain KMM 329(T), was isolated from a deep-sea sponge specimen from the Philippine Sea and subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis showed that strain KMM 329(T) clustered with the species of the genus Lysobacter. The highest level of 16S rRNA gene sequence similarity (97.

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Article Synopsis
  • A marine bacterium called "Pseudoalteromonas januaria" produces unique cyclic lipodepsipeptides labeled A/A', B/B', and C/C', with variations in their molecular structure.
  • One of the peptides (B/B' and C/C') contains a bromine atom, while peptide A/A' does not, although all peptides share a common amino acid sequence with differing C-terminal and acyl characteristics.
  • Advanced techniques like mass spectrometry and nuclear magnetic resonance spectroscopy were used to analyze and determine the structures of these peptides, highlighting the efficiency of tandem nano-electrospray ionization mass spectrometry for studying rare lipopeptides.
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Stenotrophomonas maltophilia is known to be of significance as opportunistic pathogen as well as a source of biocontrol and bioremediation activities. S. maltophilia strains have been isolated from rhizospheres, soil, clinical material, aquatic habitats, but little is known about Stenotrophomonas strains recovered from marine environments.

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The primer systems for the PCR detection of four house-keeping genes of bartonellae in clinical material were developed and tested. The tactics of the species RFLP typing was also developed and tested. The scheme of the species RFLP typing of bartonellae was tested using as an example two strains for the first time isolated in Russia from patients with endocarditis and fever of uncertain origin.

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  • An aerobically grown bacteria, strain KMM 3046(T), was isolated from a deep-sea brittle star in the Fiji Sea, showing distinctive yellow pigments.
  • The bacterium can thrive in a temperature range of 5-32°C and salt concentrations of 1-12% NaCl, with specific fatty acids and menaquinones distinguishing its biochemical profile.
  • Phylogenetic analysis indicates that it is a new genus and species named Marixanthomonas ophiurae within the Flavobacteriaceae family, with its type strain being KMM 3046(T).
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An aerobic, Gram-negative, yellow-pigmented, non-motile bacterium, designated strain KMM 3882T, was isolated from a marine bivalve (Anadara broughtoni) collected from Peter the Great Bay, Sea of Japan, and was subjected to phenotypic and phylogenetic analyses. Strain KMM 3882T was found to exert a remarkable inhibitory activity against a number of Gram-positive micro-organisms. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KMM 3882T within the genus Sphingomonas, as an independent lineage adjacent to Sphingomonas dokdonensis DS-4T and Sphingomonas panni DSM 15761T.

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Results of registration trial of combination vaccine for prevention of hepatitis A and B are presented. The trial was conducted in 5 centers of Russia in 2004-2005 with full accordance to good clinical practice requirements and standards for multicenter open randomized trials. Immunogenicity of studied combination vaccine Twinrix was evaluated in comparison with two simultaneously administered monovalent vaccines against hepatitis A and B (Havrix and Engerix-B) in 200 healthy subjects aged 18-40, which were seronegative to hepatitis A and B.

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A Gram-negative bacterium, designated strain LMG 22510T, was isolated from water of a pharmaceutical company steam generator. The cells had a ring-like and horseshoe-shaped morphology and possessed gliding motility. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was a member of the Flexibacter group within the phylum 'Bacteroidetes'; its nearest neighbour was Spirosoma linguale (88.

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