Efficient delivery of the immunodominant genes of African swine fever virus by adeno-associated virus serotype 2.

Background And Aim: Adeno-associated virus serotype 2 (AAV2) represents a promising basis for developing a virus-vector vaccine against African swine fever (ASF). This study aimed to create genetic constructs based on AAV2 to deliver the immunodominant genes of ASF virus (ASFV) and to evaluate their functionality . The efficiency and specificity of transgene expression, as well as their non-toxicity in cells of target animals, were evaluated.

Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection.

To obtain fiber materials with pronounced chemical-biological protection, metal (Zn or Ta) nanoparticles were jointly applied with polyelectrolyte complexes of enzymes and polypeptides being their stabilizers. Computer modeling revealed the preferences between certain polyelectrolyte partners for -acyl-homoserine lactone acylase and hexahistidine-tagged organophosphorus hydrolase (His-OPH) possessing the quorum quenching (QQ) behavior with bacterial cells. The combinations of metal nanoparticles and enzymes appeared to function better as compared to the combinations of the same QQ-enzymes with antibiotics (polymyxins), making it possible to decrease the applied quantities by orders of magnitude while giving the same effect.

Metal Nanoparticles for Improving Bactericide Functionality of Usual Fibers.

A wide variety of microbiological hazards stimulates a constant development of new protective materials against them. For that, the application of some nanomaterials seems to be very promising. Modification of usual fibers with different metal nanoparticles was successfully illustrated in the work.

Effect of concentrated light on morphology and vibrational properties of boron and tantalum mixtures.

Heating a mixture of boron (impurities: carbon ∼ BC, boric acid - HBO) and tantalum (Ta) powders in nitrogen flow in a xenon high-flux optical furnace was performed. As-received powder composed of h-BN, HBO, TaB, BH and a number of other phases including β-rhombohedral boron, apparently, heavily doped with Ta. FT-IR examination of any sample of the material reveals the complicated vibration spectrum containing, in particular, an absorption band near 2260 cm.

[Health-care provision to the rescue and other emergency operations in case of radiation accidents at facilities under FMBA of Russia service].

The article deals with the main tasks of health-care provision to the rescue and other emergency operations (ROEO) and organization of medical evacuation system FMBA of Russia in case of radiation accidents. The risk (threat) for workers engaged in ROEO, depending on the level of exposure of the radiation factor, is analyzed and recommendations for protective measures are considered. The authors showed the important role of the practical skills and knowledge of staff of institutions of FMBA of Russia and emergency response teams of radiation-hazardous facilities for address issues of healthcare provision to ROEO.

Medical and radiological aspects of emergency preparedness and response at SevRAO facilities.

Regulatory cooperation between the Norwegian Radiation Protection Authority and the Federal Medical Biological Agency (FMBA) of the Russian Federation has the overall goal of promoting improvements in radiation protection in Northwest Russia. One of the projects in this programme has the objectives to review and improve the existing medical emergency preparedness capabilities at the sites for temporary storage of spent nuclear fuel and radioactive waste. These are operated by SevRAO at Andreeva Bay and in Gremikha village on the Kola Peninsula.

[Results of surgical treatment in patients with drug-resistant tuberculosis].

Analysis of the results of surgical treatment in 105 patients with pulmonary tuberculosis revealed that 25 patients had postoperative complications. Complications, outcomes, dysfunctions, and life quality were analyzed in late postoperative periods after 4 types of operations: segmentectomy (n = 52), lobectomy (n = 26), pulmonectomy (n = 12), palliative operations (n = 15). The best immediate and long-term results were achieved after segmentectomy in patients with antibiotic-sensitive forms of MBT.

[The role of adoptive immunotherapy in the combined treatment of neglected forms of colorectal cancer (a preliminary report)].

Results of treatment of 24 patients with neglected colorectal tumors in the period from 1988 till 1994 are presented. Six patients had stage C and 18 patients had stage D after Dukes. Methods of combined action on the tumor including dissection of the tumor and its metastases, using immunoadoptive therapy as extracorporal connection with the donor porcine spleen, intravenous infusions of interleukin-2 and chemotherapy were employed.

[A first trial of the use of human recombinant interleukin (rIL-2) in patients with tumorous diseases].

Clinical approbation of human recombinant yeast human interleukin-2 (rIL-2) was carried out in 10 patients with III-IV stages of tumor that have undergone 65 intravenous drop by drop infusions of the drug as a course of 5-11 injections in the dosage of 1-8 mln/un. The drug toxicity was shown in 4 mln and especially, in 8 mln/un dose administration. That's why the dose of 1-2 mln/un is recommended.

[The effect of multiple and combined shock-inducing trauma on the processes of bone tissue reparation and on the recovery of work capacity in the victims].

Terms of consolidation of fractures and working ability were studied in 273 patients. Patients with multiple fractures of bones and with fractures associated with cranio-cerebral traumas were found to have slow consolidation (in 66.41%) or formation of a false joint (in 6.

[The plastic repair of extensive wound defects with vascularized flaps in treating tumors of the skin and soft tissues].

The study was concerned with comparison of the results of application of two procedures of plastic surgery using vascularized flaps for treatment of localized recurrent malignant tumors of the skin, soft tissues of the head, arms, legs and the trunk. According to one procedure (28 patients), large wound defects caused by extensive excision of tumor were treated using tissue flaps and microvascular anastomosis. In the other group of 10 patients, large defects were treated with skin-muscular flaps sitting on a fixed vascular-neural pedicle.

The extracorporeal joining of donor (pig) spleen (EJDS) for the treatment of patients with the complications of shockogenic trauma.

Introduction: A principal cause of death following presumed recovery from an episode of shock is the development of shockogenic trauma [post-resuscitation syndrome]. The causes of this complication remain unclear and its various treatments continue to be controversial.

Hypothesis: The use of perfusion of the blood of patients suffering shockogenic trauma through a donor pig spleen will decrease the mortality from the complications of this process.

[Problem of evaluation of new economic mechanism in public health (data from the Kuibyshev district)].

The two-stage sociological survey of public opinion has become an integral and, as the whole course of work showed, important part of the introduction of new economic mechanism into public health with a view of its optimization. However, it should be stressed that such a survey of public opinion is only one part of our complex programme. It seems to us that the most prospective survey appears to be the study of psychological aspects of management under new economic conditions.

[Diagnosis and treatment of mesenchymal chondrosarcoma].

Eight cases of mesenchymal chondrosarcoma (3 males and 5 females aged 15-65) are described. Morphological examination revealed in all cases biphasal tumor consisting of the chondroid tissue of varying maturity and undifferentiated tissue. Combination and complex treatment was given to 6 patients.

[Fractures of upper extremities and their treatment in polytrauma].

Under study were results of treatment of 150 patients with multiple and associated injuries of the upper extremities. Shock took place in 44% of the patients. Early fixation of the fractures of the upper extremities allowed a successful treatment of other injuries as well.

[Treatment of complicated fractures of the thoracic and lumbar segments of the spine].

A modified method of skeletal traction by the pelvis and bones of the lower extremities was used in 18 patients with the complicated trauma of the thoracic and lumbar parts of the spine. The method was shown to be highly efficient to prevent decubitus and facilitated management of the patients resulting in a simultaneous correction and fixation of the injured segments of the spine.

Laser-acupuncture reduces cigarette smoking: a preliminary report.

The stop-smoking campaign constitutes a component of the cardiovascular and oncology disease-prevention programme. In the study, laser-acupuncture was used in heavy smoking elderly patients with peripheral vascular diseases, as well as in those with lung cancer, to relieve the nicotine abstinence symptoms at the preoperational management stage. To produce a stable negative response to nicotine there was additionally applied steel needle (Seirlin-Kasei & Co.