Herpes simplex virus-specific hybridoma cells cannot be persistently infected with this virus.

Mouse hybridoma clones were examined for their ability to support replication of herpes simplex virus (HSV). Infection of hybridoma clone 1 cells producing an antibody not specific for HSV resulted in a persistent infection with a continuous production of infectious virus, whereas infection of the parental myeloma cells X63-Ag8.653 led to an abundant virus production and extinction of the culture.

Development of the rabbit retina: II. Müller cells.

Müller (glial) cells of the rabbit retina were stained with antibodies against the intermediate filament protein vimentin in retinal wholemounts from various developmental stages. Both the density of stained profiles and the mean diameter of these profiles were measured, with the microscope focus in the inner plexiform layer of the retinae. Within this retinal layer, every Müller cell possesses one stout vitread process; thus counts of the stained profiles allow an estimation of their number.

Development of the rabbit retina. I. Size of eye and retina, and postnatal cell proliferation.

Measures of rabbit eyes and retinal wholemounts were used to evaluate the development of retinal area and shape. The retina is shown to have a horizontal axis about a third longer than the vertical axis just before birth, and to adopt an almost symmetrical shape during postnatal development to adulthood. In general, retinal thickness is shown to decrease after birth, but differently in particular retinal regions: the reduction is marked in the periphery, and less pronounced in the visual streak.

[Detection of dexamethasone in horses].

Due to their marked antiinflammatory effect, synthetic corticosteroids are used to mask illness, especially lameness in horses. The detection of these drugs in equine body fluids requires accurate methods, particularly where misuse of corticosteroids is suspected. Gas chromatography/mass spectrometry (GC/MS) is well established as a reliable technique for the identification of drugs in biological fluids.

Atrial natriuretic peptide precursor material in rat testis.

Atrial natriuretic peptide (ANP) has recently been suggested to play a role in the regulation of male reproduction. Specifically, it has been shown to affect steroidogenesis in Leydig cells. The data presented here demonstrate that the rat testis also contains immunoreactive, ANP-like material (IR-ANP).

[Drug residues in food fish].

The present paper reviews different possibilities of pharmacolegal residues in fish. Sometimes the treatment of fish with registered drugs in therapeutical dosage is provable in spite of keeping the withdrawal time. To that experiences of the persistence and values of Chloramphenicol, Chlortetracycline and Furazolidone proved after therapeutical treatment in fish correlated to the water temperature are compared with results of experiments documented in literature.

Detection of latent thymidine kinase-deficient herpes simplex virus in trigeminal ganglia of mice using the polymerase chain reaction.

Latency of thymidine kinase-negative mutants of herpes simplex virus (TK- HSV) could not be detected by reactivating the virus from the ganglia of infected mice. Because Southern blot hybridization was not sensitive enough to detect viral DNA, positive results obtained by dot blot hybridization were ascertained by the highly specific and sensitive polymerase chain reaction (PCR), which detected both latent TK- HSV type 1 and 2 DNA from the trigeminal ganglia of infected mice.

[Modulation of granulocyte phagocytosis].

Non-specific complications are encountered relatively often in patients with below normal granulocytic phagocytosis rates. We tested vitamin C, transfer factor, gamma-globulin, BCG, PPD, UV irradiation of the subject's blood (UVB) and hyaluronidase with the aim of stimulating granulocytic phagocytosis with rice starch in autologous serum. In our experiments, only the addition of hyaluronidase caused any increase in phagocytotic activity.

Comparison of different methods for detecting human immune deficiency virus in human immunodeficiency virus-seropositive hemophiliacs.

Since the detection of antibodies against the human immune deficiency virus (HIV) does not definitely prove HIV infection in hemophiliacs, virus detection was attempted by virus isolation from the peripheral blood monocytes (PBL), by demonstration of p24 antigen and decline of p24 antibody, and by detection of viral DNA by the polymerase chain reaction (PCR). Virus isolation was optimized by immediate coculture of PBL and by replacement of the reverse transcriptase test by the p24 antigen test, whereas the elimination of CD8+ lymphocytes proved to be unnecessary. Virus detection was dependent on the clinical stage of the illness.

Immunoreactive atrial natriuretic peptide in the guinea pig spleen.

The presence of immunoreactive ANP precursor-like material in the guinea pig spleen is suggested. This is based on the following experimental evidence: An acidic extract of guinea pig spleen analysed by Sephadex G-50 gelfiltration contained 4.6 pmol/g wet tissue immunoreactive atrial natriuretic peptide (IR-ANP), coeluting with the 15 kDa synthetic ANP (2-126).

Presence of atrial natriuretic peptide-like material in guinea pig intestine.

Acidic extracts of guinea pig jejunum and colon contain atrial natriuretic peptide-like material (IR-ANP) detected by radioimmunoassay after purification by Sephadex G-50 gel filtration. Immunohistochemical analysis of guinea pig colon also revealed IR-ANP to be located directly beneath the lamina muscularis mucosae. High performance gel permeation chromatography (HP-GPC) and reverse phase high performance chromatography (RP-HPLC) of the IR-ANP showed correspondence to the 15 kD ANP precursor molecule (pro-ANP).

[Clinical and prognostic aspects of multiple cancers].

The anamnestic data, course and prognosis for 76 patients with multiple carcinomas were statistically analyzed in a retrospective study undertaken jointly with the oncological working group. The importance of multidisciplinary care for this group of patients over a period exceeding five years must be emphasized. If the general status of a patient deteriorates, the doctor should always consider the possibility of a second or third carcinoma.

Immunological mechanisms giving rise to latency of herpes simplex virus in the spinal ganglia of the mouse.

In the model of genital herpes simplex virus (HSV)-infection of mice, early latency could be induced by passive immunization with HSV-specific antibodies and, to a lesser degree, by adoptive transfer of immune lymphocytes prepared from spleen and draining lymph nodes of genitally infected syngeneic mice. Conversely, spontaneously occurring latency was inhibited by treatment of the animals with cyclophosphamide (Cph) and, to a lesser degree, with cyclosporin A (CyA). Whereas the effect of CyA could be compensated by passively administered HSV-specific antibodies, that of Cph could not.

[Oncologic after-care of patients with prostate cancer].

The aim of the examinations is the proof of the clinical value of the granulocyte phagocytosis test in the after-care of the palliatively treated patients with a carcinoma of the prostate. On the basis of the analysis of the own patient and the observation of the course the routine control of the granulocyte phagocytosis is recommended, since unspecific complication are recognized earlier and thus may be treated better.

[A foreign body in the urinary bladder].

A case concerning the problems of the foreign bodies of the urinary bladder is demonstrated. The 6-year indwelling stay of the foreign body without consultation of a physician and the oral intake at first reported, which was later on revised, are unusual.

[Simultaneous investigations on granulocyte phagocytosis using rice starch and on granulocyte migration--a study in the preoperative definition of the defense situation of the organism].

Information which is more extensive than the unspecific value of the granulocytophagocytosis could not be found out by the simultaneous investigations of the granulocytophagocytosis and the granulocytomigration. The application of AHLG and tumor-associated antigens in the migration test system did not show specific results for the oncology. The granulocytomigration in a home-made modified Boyden chamber did not show any sexual distinctions.

[Significance of preoperative prognosis].

The granulocyte phagocytosis test, Karnofsky index, and ASA standard were used in the context of 287 patients for general surgery, for the purpose of retrospective elucidation of the relationship between presurgical prognostication and postsurgical complications. Sensitivity, specificity, and "predictive values" were calculated. The highest information potential in terms of prognostication was recorded from objectively measurable granulocyte phagocytosis.

Flow cytometric measurements of phagocytosis. I. A methodical and comparative study.

A flow cytometric technique is described for measurement of phagocytic activity in human leucocytes using FITC-labelled latex particles (0.77 micron). Cell suspensions separated from peripheral blood by dextran sedimentation were hemolyzed to avoid particle uptake by erythrocytes.

[Modification of granulocyte phagocytosis by extracts from tumor antigens].

The results with the granulocyte phagocytosis test adding various tumor antigen preparations are represented. In comparison with clinically healthy probands and patients having benign diseases there could not be proved any malignancy and organ specificity of granulocyte phagocytosis in patients with malignant tumors of different stages and various localization of organs.