Optimization of Δ-tetrahydrocannabinolic acid synthase production in Komagataella phaffii via post-translational bottleneck identification.

Δ-Tetrahydrocannabinolic acid (THCA) is a secondary natural product from the plant Cannabis sativa L. with therapeutic indications like analgesics for cancer pain or reducing spasticity associated with multiple sclerosis. Here, we investigated the influence of the co-expression of 12 helper protein genes from Komagataella phaffii (formerly Pichia pastoris) on the functional expression of the Δ-tetrahydrocannabinolic acid synthase (THCAS) heterologously expressed in K.

Engineering yeasts as platform organisms for cannabinoid biosynthesis.

Δ-tetrahydrocannabinolic acid (THCA) is a plant derived secondary natural product from the plant Cannabis satival. The discovery of the human endocannabinoid system in the late 1980s resulted in a growing number of known physiological functions of both synthetic and plant derived cannabinoids. Thus, manifold therapeutic indications of cannabinoids currently comprise a significant area of research.