N-Amino Norbornene Imides as Scalable Monomers for Living Ring-Opening Metathesis Polymerization.

Ring-opening metathesis polymerization (ROMP) is a well-established polymerization method that uses strained cyclic olefins to produce polymers with controlled molecular weight and dispersity suitable for aggregation in solution, leading to polymerization induced self-assembly (PISA). Herein, we report new norbornene-based monomers for the living ROMP that were synthesized on the multigram scale (20 g) starting from -5-norbornene--2,3-dicarboxylic anhydride and hydrazine. Upon further reaction with carboxylic acid chlorides, non-hydrogen-bond-forming derivatives could be obtained.

DDX37 and DDX50 maintain genome stability by preventing transcription-dependent R-loop formation.

R-loops consist of an RNA-DNA hybrid and a displaced single-stranded DNA strand that play a central role in several biological processes. However, as the presence of aberrant R-loops forms a significant threat to genome stability, R-loop formation and resolution is strictly controlled by RNAse H and helicases. In a screening for RNA helicases, previously described as RNA-DNA hybrid interactors, that control genome integrity, we identified for the first time DDX37 and DDX50.

Capnometry-Guided Respiratory Intervention in Veteran PTSD: Impact on Symptom Clusters.

Post-traumatic stress disorder (PTSD) is a challenging psychiatric condition to treat, with suboptimal recovery and difficulty tolerating exposure-based psychotherapies often noted in outcomes research. The aim of this study was to examine patterns of symptom reduction in veterans with PTSD treated with a Capnometry-Guided Respiratory Intervention (CGRI), a 28-day treatment teaching about the normalization of respiratory rate and exhaled carbon dioxide levels via biofeedback. We hypothesized reductions in total PCL-5 scores and all symptom clusters immediately post-treatment but with relative resistance to changes in hyperarousal symptoms, as reported in the outcomes of research using other evidence-based psychotherapies.

Biomechanical Insights into the Proteomic Profiling of Cells in Response to Red Light Absorption.

Photobiomodulation (PBM) is a promising non-invasive therapy for tissue repair, but its underlying cellular mechanisms are not fully understood. In this study, the biomechanical and proteomic responses of three cell types - keratinocytes (HACAT), fibroblasts (L929), and osteoblasts (OFCOLII) - exposed to red light (633 nm) are investigated using atomic force microscopy (AFM) and mass spectrometry-based proteomic analysis. Red light absorption resulted in cell-type-specific changes in viscoelastic properties, with fibroblasts exhibiting increased fluidity, reduced stiffness, and enhanced motility.

study of essential oils encapsulated in chitosan microparticles against and their pathogenicity in .

Histoplasmosis, caused by , poses risks for immunocompromised individuals. With limited therapeutic options, this study explores microparticles as antimicrobial delivery systems for and essential oils against . The broth microdilution assay showed MICs of 32 to 128 µg/mL in filamentous phase and 8 to 64 µg/mL in yeast phase.

Pharmacogenetic Testing in Treatment-resistant Panic Disorder: a Preliminary Analysis.

Background: Many pharmacological treatments are considered effective in the treatment of panic disorder (PD), however, about 20 to 40% of the patients have treatment-resistant PD. Pharmacogenetics could explain why some patients are treatment-resistant.

Objective: Our objective was to gather preliminary data on the clinical usefulness of pharmacogenetic testing in this disorder.

Mobile Application for Tracking Children with Autistic Spectrum Disorder: Content Validation and Usability.

Background: This study aims to validate the content and evaluate the usability of the Autism App for screening children with autism spectrum disorder.

Methods: This methodological study was conducted between August 2023 and March 2024. The study included 15 experts for content validation and nine for usability evaluation, all of whom had experience in the health/technology field.

Biochemical analysis to study wild-type and polyglutamine-expanded ATXN3 species.

Spinocerebellar ataxia type 3 (SCA3) is a cureless neurodegenerative disease recognized as the most prevalent form of dominantly inherited ataxia worldwide. The main hallmark of SCA3 is the expansion of a polyglutamine tract located in the C-terminal of Ataxin-3 (or ATXN3) protein, that triggers the mis-localization and toxic aggregation of ATXN3 in neuronal cells. The propensity of wild type and polyglutamine-expanded ATXN3 proteins to aggregate has been extensively studied over the last decades.

The acute effect of bilateral cathodic transcranial direct current stimulation on respiratory muscle strength and endurance.

Introduction: Transcranial direct current stimulation (tDCS) is a non-invasive technique with therapeutic potential, especially in respiratory muscle training (RMT) in pathological conditions such as chronic obstructive pulmonary disease and heart failure.

Objective: To evaluate the effect of bilateral cathodic tDCS on respiratory muscle strength and endurance in healthy young and elderly women.

Methods: An experimental, randomized study with 80 participants divided into young and old women, subdivided into intervention and sham control groups.

Lobatozoum woodi n. sp. (Digenea: Didymozoidae) parasitizing Euthynnus alletteratus (Scombriformes: Scombridae) in the coast of the State of Rio de Janeiro, Brazil.

The aim of this study was to describe a new species of Didymozoidae (Trematoda) found in the mesentery of Euthynnus alletteratus (Rafinesque) off Cabo Frio, in the coastal area of the state of Rio de Janeiro, Brazil. Thirty specimens of E. alletteratus were obtained between August 2023 and June 2024, directly from traders who sell fresh fish in markets.

Nanostructure Formation in Glycerolipid Films during Enzymatic Hydrolysis: A GISAXS Study.

Responsive nanostructured films from food-grade lipids can be valuable for food, pharmaceutical, and biotechnological science. Lyotropic liquid crystalline structures that respond to enzymes in their environment can, for instance, be innovated as drug delivery platforms or biosensors. However, the structural changes that such films undergo during enzymatic reactions with lipase are not yet understood.

Successful Closure of an Idiopathic Macular Hole With Topical Therapy After Failed Surgery.

To describe the successful closure of an idiopathic macular hole (MH) with topical therapy after surgery failed. A case report was evaluated and prospective literature review performed. After surgery to correct an MH in a 57-year-old female patient failed, topical therapy was initiated.

ATXN3: a multifunctional protein involved in the polyglutamine disease spinocerebellar ataxia type 3.

ATXN3 is a ubiquitin hydrolase (or deubiquitinase, DUB), product of the gene, ubiquitously expressed in various cell types including peripheral and neuronal tissues and involved in several cellular pathways. Importantly, the expansion of the CAG trinucleotides within the gene leads to an expanded polyglutamine domain in the encoded protein, which has been associated with the onset of the spinocerebellar ataxia type 3, also known as Machado-Joseph disease, the most common dominantly inherited ataxia worldwide. ATXN3 has therefore been under intensive investigation for decades.

Repetitive Transcranial Magnetic Stimulation (rTMS) in Major Depression.

Major depressive disorder (MDD) is a psychiatric disorder with several effective therapeutic approaches, being antidepressants and psychotherapies the first-line treatments. Nonetheless, due to side effects, limited efficacy, and contraindications for these treatments, alternative treatment options are required. Neurostimulation is a non-pharmacological and non-psychotherapeutic approach that has been under study for diverse neuropsychiatric conditions in the form of electrical or magnetic stimulation of the brain.

Photoluminescence and magnetic properties of isostructural europium(III), gadolinium(III) and terbium(III) oxamate-based coordination polymers.

Developing and investigating advanced multifunctional materials with magnetic properties as candidates for assembling spin qubits for quantum computing is imperative. A new polytopic ligand based on oxamate and aniline was used to promote the synthesis of three neutral homometallic lanthanide-coordinated polymers. New complexes with the formula {Ln(phox)(DMSO)(HO)}, where Ln = Eu (1), Gd (2), and Tb (3) [phox = -(phenyl)oxamate and DMSO = dimethylsulfoxide], were synthesized and well characterized by spectroscopic methods as well as X-ray crystallographic analysis.

Neurochemical and genetic factors in panic disorder: a systematic review.

This systematic review addresses the complex nature of Panic Disorder (PD), characterized by recurrent episodes of acute fear, with a focus on updating and consolidating knowledge regarding neurochemical, genetic, and epigenetic factors associated with PD. Utilizing the PRISMA methodology, 33 original peer-reviewed studies were identified, comprising 6 studies related to human neurochemicals, 10 related to human genetic or epigenetic alterations, and 17 animal studies. The review reveals patterns of altered expression in various biological systems, including neurotransmission, the Hypothalamic-Pituitary-Adrenal (HPA) axis, neuroplasticity, and genetic and epigenetic factors leading to neuroanatomical modifications.

Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy.

Understanding tumor-host immune interactions and the mechanisms of lung cancer response to immunotherapy is crucial. Current preclinical models used to study this often fall short of capturing the complexities of human lung cancer and lead to inconclusive results. To bridge the gap, we introduce two new murine monoclonal lung cancer cell lines for use in immunocompetent orthotopic models.

FeCo@hydrochar nanocomposites as efficient peroxymonosulfate activator for organic pollutant degradation.

Sulfate radical-based advanced oxidation processes (SR-AOPs) are renowned for their exceptional capacity to degrade refractory organic pollutants due to their wide applicability, cost-effectiveness, and swift mineralization and oxidation rates. The primary sources of radicals in AOPs are persulfate (PS) and peroxymonosulfate (PMS) ions, sparking significant interest in their mechanistic and catalytic aspects. To develop a novel nanocatalyst for SR-AOPs, particularly for PMS activation, we synthesized carbon-coated FeCo nanoparticles (NPs) using solvothermal methods based on the polyol approach.

Crucial role of the NSE1 RING domain in Smc5/6 stability and FANCM-independent fork progression.

The Smc5/6 complex is a highly conserved molecular machine involved in the maintenance of genome integrity. While its functions largely depend on restraining the fork remodeling activity of Mph1 in yeast, the presence of an analogous Smc5/6-FANCM regulation in humans remains unknown. We generated human cell lines harboring mutations in the NSE1 subunit of the Smc5/6 complex.

Physical Capacities and Combat Performance Characteristics of Male and Female Olympic Boxers.

: The study characterized the anthropometrical and cardiorespiratory profile, and the cardiorespiratory, bio-chemical and immunological responses to 3 × 3 min round (R) free-contact/combat boxing simulation, in elite Olympic Boxers (4 female and 10 male). : The evaluation consisted of resting metabolic rate, anthropometric measurement, maximal graded test exercise (visit 1), free combat simulation (3 × 3 min R, 1 minute rest), and blood samples collected before, during and after the combat (visit 2). : Respectively, females and males had (mean±SD; or median: for non-parametric data) body fat percentage (17.

Measuring the viscoelastic relaxation function of cells with a time-dependent interpretation of the Hertz-Sneddon indentation model.

The Hertz-Sneddon elastic indentation model is widely adopted in the biomechanical investigation of living cells and other soft materials using atomic force microscopy despite the explicit viscoelastic nature of these materials. In this work, we demonstrate that an exact analytical viscoelastic force model for power-law materials, can be interpreted as a time-dependent Hertz-Sneddon-like model. Characterizing fibroblasts (L929) and osteoblasts (OFCOLII) demonstrates the model's accuracy.

Age-Related Changes in Survival Behaviour in Parasite-Free Hatchery-Reared Rainbow Trout ().

Millions of hatchery-reared Rainbow trout are currently released in Australian waters to support recreational fisheries objectives, yet many of these fish die soon after release. In addition, little is known whether these fish harbour parasites that can potentially threaten freshwater ecosystems and human health. Here, we tested the behaviour of hatchery-reared trout using six tank-based tests at six different ages to evaluate their chances of survival and then dissected fish to investigate parasite prevalence.

Advances in surface design and biomedical applications of magnetic nanoparticles.

Despite significant efforts by scientists in the development of advanced nanotechnology materials for smart diagnosis devices and drug delivery systems, the success of clinical trials remains largely elusive. In order to address this biomedical challenge, magnetic nanoparticles (MNPs) have gained attention as a promising candidate due to their theranostic properties, which allow the simultaneous treatment and diagnosis of a disease. Moreover, MNPs have advantageous characteristics such as a larger surface area, high surface-to-volume ratio, enhanced mobility, mass transference and, more notably, easy manipulation under external magnetic fields.