Publications by authors named "Frederick S Ditmars"
Article Synopsis
- Hard-to-heal wounds, particularly diabetic and venous leg ulcers, pose significant challenges to patients and the healthcare system due to their resistance to standard treatments.
- A multicenter retrospective study showed that the application of dehydrated amniotic membrane (DHAM) led to substantial reductions in wound size, with 50% size reduction occurring after roughly two applications.
- The study is the first to compare DHAM efficacy specifically for these difficult-to-treat wounds, suggesting that its use could enhance healing outcomes and reduce clinic visits.
Article Synopsis
- Amniotic fluid (AF) is crucial for fetal development and contains regenerative progenitor cells, but recent research highlights the benefits of cell-free amniotic fluid (cfAF), which is made up of soluble components and extracellular vesicles.
- Studies show that cfAF can enhance healing by promoting immunomodulatory, anti-inflammatory, and pro-growth effects on target cells, and it helps restore cells to a normal state after stress or disease.
- Although the exact mechanisms of these effects are still being explored, cfAF has shown promise in treating skin wounds and possibly joint conditions, suggesting it has broader therapeutic applications pending further research.
Chronic, non-healing venous ulcers of the lower extremity are often limb-threatening conditions. Their management is characterized by a prolonged and frequently frustrating clinical course that represents an economic burden to both the patient and healthcare system. During the last two decades, thermal ablation of underlying incompetent venous systems has been extensively utilized to treat chronic venous insufficiency.
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- - The study investigates the role of alternative splicing in the development of the three embryonic germ layers using RNA-Seq on human embryonic stem cells and their derived lineages, identifying distinct splicing programs for each lineage.
- - Significant differences in splicing were found primarily between definitive endoderm and cardiac mesoderm, with an integrative analysis revealing RNA binding proteins that regulate these splicing events, particularly highlighting Quaking (QKI) in cardiac mesoderm specification.
- - QKI knockout led to disruptions in the cardiac mesoderm splicing program and the formation of myocytes by reducing splice variants of the BIN1 gene, emphasizing QKI's role in managing this process through specific RNA interactions and chromatin
Background: Tibial stress fractures are not uncommon in pediatric athletes. The severity of injury may be graded using magnetic resonance imaging (MRI).
Objective: To determine whether Fredericson MRI grading of tibial stress fractures can differentiate times to recovery across different grades in pediatric athletes.
Benzoic acid, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline during the experimental evolution of K-12. Transcriptomes of isolates evolved with benzoate showed the reversal of benzoate-dependent regulation, including the downregulation of multidrug efflux pump genes, the gene for the Gad acid resistance regulon, the nitrate reductase genes , and the gene for the acid-consuming hydrogenase Hyd-3. However, the benzoate-evolved strains had increased expression of OmpF and other large-hole porins that admit fermentable substrates and antibiotics.
View Article and Find Full Text PDFUnlabelled: Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5, a pH at which permeant acids concentrate in the cytoplasm.
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