Publications by authors named "Frederick J Awuku"

Introduction: The cowpea weevil, Fab., is the most economically important storage pest of cowpeas, causing up to 100 percent grain losses within six months of storage. To sustainably resist weevil damage, the cowpea cultivar IT86D-1010 was genetically modified via -mediated transformation to produce event CSI-32, which expresses the kidney bean alpha-amylase inhibitor 1 (αAI-1) protein exclusively in the seed, providing suppression of weevil development.

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Article Synopsis
  • - The cowpea aphid is a significant pest affecting cowpea crops in West Africa, necessitating farmer control, especially during the vegetative stage, making aphid-resistant crop varieties a valuable alternative to costly insecticides.
  • - Researchers developed a new cowpea variety, Zaayura, by using gene pyramiding techniques to incorporate two aphid-resistance genes from different sources through marker-assisted backcrossing, enhancing its robustness against aphid infestations.
  • - After multiple backcross generations, five improved lines of Zaayura were created, showing high recurrent parent genome recovery and superior resistance to cowpea aphids, indicating promising results for sustainable farming practices.
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  • The study analyzes the genetic diversity and population structure of 242 newly developed maize inbred lines using 3,305 SNP markers to understand their genetic makeup and potential for host plant resistance.
  • It found that the inbred lines showed high gene diversity and low levels of heterozygosity, indicating they are distantly related with unique genetic traits, which could be valuable for maize improvement.
  • The results also suggest that these inbred lines can be categorized into three subgroups, providing insights into their breeding history and assisting in the development of locally adapted varieties resistant to fall armyworm in West and Central Africa.
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Development of high yielding cowpea varieties coupled with good taste and rich in essential minerals can promote consumption and thus nutrition and profitability. The sweet taste of cowpea grain is determined by its sugar content, which comprises mainly sucrose and galacto-oligosaccharides (GOS) including raffinose and stachyose. However, GOS are indigestible and their fermentation in the colon can produce excess intestinal gas, causing undesirable bloating and flatulence.

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Macrophomina root rot disease (MRRD) caused by is an emerging threat to the profitable cowpea production in northern Ghana. Recommended control methods including the use of fungicides are ineffective and expensive for resource poor farmers whilst biocontrol options are not commercially available. An integrated method based on host plant resistance is considered the cheapest and most effective method of managing the disease.

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Information on the general combining ability of inbred lines and the specific combining ability of hybrid combinations is crucial for successful hybrid development. The objectives of this study were to (i) determine the combining ability of thirty selected early maturing maize inbred lines under -infested and optimal environments, (ii) classify the inbred lines into heterotic groups using the general combining ability effects of multiple traits (HGCAMT) and the single nucleotide polymorphism genetic distance (SNP- GD) methods, and (iii) assess the effectiveness of the heterotic grouping methods. One hundred and fifty single-cross hybrids were generated from the thirty inbred lines using the North Carolina Design II mating method.

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Global climate change is expected to further intensify the already harsh conditions in the dry savannah ecological zones of sub-Saharan Africa, posing serious threats to food and income security of millions of smallholder farmers. Breeding cowpea for improved earliness could help minimize this risk, by ensuring that the crops complete their lifecycle before the cessation of rainfall. In this study, we crossed two sets of cowpea lines showing contrasting phenotypes for earliness in terms of days to 50% flowering (DFF).

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Information on genetic diversity and population structure are very important in any breeding programme for the improvement of traits of interest and the development of outstanding products for commercialization. In the present study, we assessed the genetic diversity of 94 early-maturing white and yellow tropical maize inbred lines using single nucleotide polymorphism (SNP) markers. The larger number of SNP markers used in this study allowed a clearer inference of the population structure of the 94 inbred lines.

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