Publications by authors named "Frederic Lemaire"
The ability to specifically engineer the genome of living cells at precise locations using rare-cutting designer endonucleases has broad implications for biotechnology and medicine, particularly for functional genomics, transgenics and gene therapy. However, the potential impact of chromosomal context and epigenetics on designer endonuclease-mediated genome editing is poorly understood. To address this question, we conducted a comprehensive analysis on the efficacy of 37 endonucleases derived from the quintessential I-CreI meganuclease that were specifically designed to cleave 39 different genomic targets.
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- The text discusses advancements in chemical hazard management and the need for efficient in vitro toxicity assays to reduce animal testing, particularly in the context of the REACH policy and drug ADMETOX prediction.* -
- A new in vitro method was developed using a specialized cell line that responds to stress, allowing for the testing of various toxins, and successfully identifying chemical-specific responses and calculating IC50 values that align with existing data.* -
- The findings emphasize the importance of monitoring cell stress over traditional mortality measures, showcasing a new generation of high-throughput assays that can effectively evaluate chemical hazards while adhering to regulatory frameworks.*
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer among men in the developed world. There is a need, for both clinical and scientific reasons, to find markers to identify patients with aggressive disease as early as possible, and to understand the events leading to malignant transformation and susceptibility to metastasis. We report the first large-scale gene expression analysis of a unique HNSCC location, the hypopharynx.
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