Mapping H in the Nanoscale (AC)-Ag Fluorophore.

When strands of DNA encapsulate silver clusters, supramolecular optical chromophores develop. However, how a particular structure endows a specific spectrum remains poorly understood. Here, we used neutron diffraction to map protonation in (AC)-Ag, a green-emitting fluorophore with a "Big Dipper" arrangement of silvers.

Defining the human kidney N-glycome in normal and cancer tissues using MALDI imaging mass spectrometry.

Clear-cell renal cell carcinoma (ccRCC) presents challenges to clinical management because of late-stage detection, treatment resistance, and frequent disease recurrence. Metabolically, ccRCC has a well-described Warburg effect utilization of glucose, but how this affects complex carbohydrate synthesis and alterations to protein and cell surface glycosylation is poorly defined. Using an imaging mass spectrometry approach, N-glycosylation patterns and compositional differences were assessed between tumor and nontumor regions of formalin-fixed clinical ccRCC specimens and tissue microarrays.

Culturally safe end-of-life care for First Nations persons living on reserve.

End-of-life (EOL) care involves not just the final few days of a person's life but also living with a terminal illness over an extended period of time. Importantly, in addition to medical care and relief of physical suffering, it focuses on quality of life, honoring personal healthcare treatment decisions, supporting the family, and psychological, cultural and spiritual concerns for dying people and their families. The goal of this commentary is to raise rehabilitation therapists' awareness of the need for culturally safe EOL care services for First Nations persons who live on reserve and to identify strategies to help resolve this unmet need.

A novel approach to a rabies vaccine based on a recombinant single-cycle flavivirus vector.

The RepliVax® vaccine (RV) platform is based on flavivirus genomes that are rationally attenuated by deletion. These single-cycle RV vaccine candidates targeting flavivirus pathogens have been demonstrated to be safe, highly immunogenic, and efficacious in animal models, including non-human primates. Here we show utility of the technology for delivery of a non-flavivirus immunogen by engineering several West Nile-based RV vectors to express full-length rabies virus G protein.