Straightforward fabrication of electrochemical aptasensors with outstanding antifouling performance.

Self-assembled monolayers (SAMs) are popular tools for many different applications - SAMs of commercially available chemicals that convincingly inhibit unspecific binding for electrochemical sensors, however, have yet to be developed. While adsorption of foulants prohibits the reliable analysis of biological samples, unspecific binding of the analyte similarly impedes the investigation of binding characteristics from buffer solutions. In this communication, diglycolamine is introduced for the modification of electrodes with outstanding antifouling performance.

Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review.

The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the biosensor has to be stable and reproducible, and highly sensitive to detect potentially extremely low concentrations and prevent the nonspecific binding of interfering compounds. In this review, we present an overview of recently published (2017-2019) immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins.