Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.

The division of labor between pulmonary phagocytic subsets [macrophage/monocyte and dendritic cell (DC) subpopulations] has been described at the functional level. However, whether these lung phagocytes also display unique spatial distribution remains unclear. Here, to analyze cellular distribution in lung compartments and contacts between phagocyte subpopulations, we established an immunohistochemistry (IHC)-based method to clearly identify murine lung phagocyte subsets based on differential expression of CD11c, CD11b, MHC-II, Langerin and mPDCA-1.