Publications by authors named "Fransje Boot"
Article Synopsis
- Access to Human Beta Cells
- : The study presents EndoC-βH5 cells as an advanced model for understanding human pancreatic beta cell functions and potential diabetes treatments, closely mimicking primary adult cells.
- Cell Generation and Features
- : These cells were created by integrating specific genes into human fetal pancreas, with successful removal of unwanted transgenes, resulting in cells that are easy to use and assess for insulin secretion and other functions.
- Findings and Applications
- : EndoC-βH5 cells demonstrate strong glucose-dependent insulin secretion and are suitable for drug testing and studying beta cell behavior, indicating their utility in diabetes research and therapy.
Insulin secretion in pancreatic β-cells is regulated by cortical complexes that are enriched at the sites of adhesion to extracellular matrix facing the vasculature. Many components of these complexes, including bassoon, RIM, ELKS and liprins, are shared with neuronal synapses. Here, we show that insulin secretion sites also contain the non-neuronal proteins LL5β (also known as PHLDB2) and KANK1, which, in migrating cells, organize exocytotic machinery in the vicinity of integrin-based adhesions.
View Article and Find Full Text PDFObjective: Environmental factors driving the development of type 1 diabetes (T1D) are still largely unknown. Both animal and human studies have shown an association between altered fecal microbiota composition, impaired production of short-chain fatty acids (SCFA) and T1D onset. However, observational evidence on SCFA and fecal and oral microbiota in adults with longstanding T1D vs healthy controls (HC) is lacking.
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