Publications by authors named "Franklin Kostas"
Article Synopsis
- Researchers developed a new method called ligation-enabled mRNA-oligonucleotide assembly (LEGO) to improve mRNA translation for protein and vaccine therapies.
- They found that using a branched mRNA cap helps initiate translation effectively, even in mRNAs that typically struggle without specific entry sites.
- By modifying the mRNA structure, they significantly boosted protein production and antibody response in the context of vaccines, showing a potential for better mRNA designs in therapies and research.
Although messenger RNA (mRNA) has proved effective as a vaccine, its potential as a general therapeutic modality is limited by its instability and low translation capacity. To increase the duration and level of protein expression from mRNA, we designed and synthesized topologically and chemically modified mRNAs with multiple synthetic poly(A) tails. Here we demonstrate that the optimized multitailed mRNA yielded ~4.
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