Investigation of PAH biomarkers in the urine of workers exposed to hot asphalt.

Airborne emissions from hot asphalt contain mixtures of polycyclic aromatic hydrocarbons (PAHs), including several carcinogens. We investigated urinary biomarkers of three PAHs, namely naphthalene (Nap), phenanthrene (Phe), and pyrene (Pyr) in 20 road-paving workers exposed to hot asphalt and in 6 road milling workers who were not using hot asphalt (reference group). Our analysis included baseline urine samples as well as postshift, bedtime, and morning samples collected over three consecutive days.

Using liquid chromatography-tandem mass spectrometry to quantify monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine.

We present an assay which employs enzyme digestion and solid phase extraction followed by liquid chromatography-tandem mass spectrometry to simultaneously quantify 16 hydroxylated polycyclic aromatic hydrocarbons (OHPAHs) in 3-ml samples of urine. The analytes consisted of 2-, 3-, and 4-ring OHPAHs, namely, 1- and 2-hydroxynaphthalene (1- and 2-OHNAP), 2-hydroxyfluorine (2-OHFLU), 1-, 2-, 3-, 4-, and 9-hydroxyphenanthrene (1-, 2-, 3-, 4-, and 9-OHPHE), 1-hydroxypyrene (1-OHPYR), 1- and 2-hydroxybenzo(a)anthracene (1- and 2-OHBAA), 3- and 6-hydroxychrysene (3- and 6-OHCHR) and 3-, 7-, and 9-hydroxybenzo(a)pyrene (3-, 7-, and 9-OHBAP). The method was validated using urine samples from steel workers and control subjects.

Synthesis and incorporation of a cationic pyridinium nucleoside mimic in duplex DNA: effect on long-distance radical cation transport.

[Structure: see text] A convenient method for the synthesis and solid-phase incorporation of a cationic nucleoside mimic and its role in DNA long-range charge transfer are described.

The reaction of water with guanosine radical cation in DNA.

Structural analyses of DNA oligonucleotides indicate the presence of strongly bound water molecules in the major and minor grooves. These water molecules may react with guanosine radical cation to form 8-oxo-7,8-dihydroguanine and other oxidation products, which can result in strand scission or other DNA damage. We probed the reaction of guanosine radical cation with water by incorporating alkyl-substituted cytidines and thymidines into anthraquinone-linked DNA duplexes and irradiating them with UV light at 350 nm.