Development of a Tool to Assess Inference-Making and Reasoning in Biology.

Making inferences and reasoning with new scientific information is critical for successful performance in biology coursework. Thus, identifying students who are weak in these skills could allow the early provision of additional support and course placement recommendations to help students develop their reasoning abilities, leading to better performance and less attrition within biology courses. Researchers across universities partnered to develop a measure to assess students' inference-making abilities in biology.

Small Ca releases enable hour-long high-frequency contractions in midshipman swimbladder muscle.

Type I males of the Pacific midshipman fish () vibrate their swimbladder to generate mating calls, or "hums," that attract females to their nests. In contrast to the intermittent calls produced by male Atlantic toadfish (), which occur with a duty cycle (calling time divided by total time) of only 3-8%, midshipman can call continuously for up to an hour. With 100% duty cycles and frequencies of 50-100 Hz (15°C), the superfast muscle fibers that surround the midshipman swimbladder may contract and relax as many as 360,000 times in 1 h.

Muscle force, work and cost: a novel technique to revisit the Fenn effect.

Muscle produces force by forming cross-bridges, using energy released from ATP. While the magnitude and duration of force production primarily determine the energy requirement, nearly a century ago Fenn observed that muscle shortening or lengthening influenced energetic cost of contraction. When work is done by the muscle, the energy cost is increased and when work is done on the muscle the energy cost is reduced.

Intracellular calcium movements during relaxation and recovery of superfast muscle fibers of the toadfish swimbladder.

The mating call of the Atlantic toadfish is generated by bursts of high-frequency twitches of the superfast twitch fibers that surround the swimbladder. At 16°C, a calling period can last several hours, with individual 80-100-Hz calls lasting ∼ 500 ms interleaved with silent periods (intercall intervals) lasting ∼ 10 s. To understand the intracellular movements of Ca(2+) during the intercall intervals, superfast fibers were microinjected with fluo-4, a high-affinity fluorescent Ca(2+) indicator, and stimulated by trains of 40 action potentials at 83 Hz, which mimics fiber activity during calling.

High efficiency in human muscle: an anomaly and an opportunity?

Can human muscle be highly efficient in vivo? Animal muscles typically show contraction-coupling efficiencies <50% in vitro but a recent study reports that the human first dorsal interosseous (FDI) muscle of the hand has an efficiency value in vivo of 68%. We examine two key factors that could account for this apparently high efficiency value: (1) transfer of cross-bridge work into mechanical work and (2) the use of elastic energy to do external work. Our analysis supports a high contractile efficiency reflective of nearly complete transfer of muscular to mechanical work with no contribution by recycling of elastic energy to mechanical work.

The metabolic power requirements of flight and estimations of flight muscle efficiency in the cockatiel (Nymphicus hollandicus).

Little is known about how in vivo muscle efficiency, that is the ratio of mechanical and metabolic power, is affected by changes in locomotory tasks. One of the main problems with determining in vivo muscle efficiency is the large number of muscles generally used to produce mechanical power. Animal flight provides a unique model for determining muscle efficiency because only one muscle, the pectoralis muscle, produces nearly all of the mechanical power required for flight.

The integration of lateral gastrocnemius muscle function and kinematics in running turkeys.

Animals commonly move over a range of speeds, and encounter considerable variation in habitat structure, such as inclines. Hindlimb kinematics and muscle function in diverse groups of vertebrates are affected by these changes in behavior and habitat structure, providing a fruitful source of variation for studying the integration of kinematics and muscle function. While it has been observed in a variety of vertebrates that muscle length change can be minimal during locomotion, it is unclear how, and to what degree, in vivo muscle length change patterns are integrated with kinematics.

Task-dependent force sharing between muscle synergists during locomotion in turkeys.

At most joints, there is a redundancy of muscle function. For any given movement, there are a wide range of possible solutions to the problem of how force is shared among muscle synergists. A better understanding of how force is shared among muscle synergists can provide insight into the mechanics and control of movement.

Relative shortening velocity in locomotor muscles: turkey ankle extensors operate at low V/V(max).

The force-velocity properties of skeletal muscle have an important influence on locomotor performance. All skeletal muscles produce less force the faster they shorten and typically develop maximal power at velocities of approximately 30% of maximum shortening velocity (V(max)). We used direct measurements of muscle mechanical function in two ankle extensor muscles of wild turkeys to test the hypothesis that during level running muscles operate at velocities that favor force rather than power.

Muscle strain is modulated more with running slope than speed in wild turkey knee and hip extensors.

We examined the length changes and electromyographic (EMG) activity of two hindlimb muscles in wild turkeys, to determine how these muscles modulate mechanical function with changes in running speed and slope. The muscles studied were the iliotibialis lateralis pars postacetabularis (ILPO), a biarticular knee and hip extensor, and the femorotibialis lateralis (FT), a knee extensor. Muscle length changes were recorded using sonomicrometry, and EMG activity was recorded from indwelling bipolar electrodes as the animals walked and ran at a range of speeds (1-3.

Mechanical function of two ankle extensors in wild turkeys: shifts from energy production to energy absorption during incline versus decline running.

We investigated the mechanical function of two ankle extensor muscles, the lateral gastrocnemius (LG) and peroneus longus (PL), in wild turkeys Meleagris gallopavo during steady speed running. We hypothesized that mechanical work output of the LG and PL during running parallels the demand for mechanical work on the body. The turkeys ran on level, inclined (+6 degrees, +12 degrees ) and declined (-6 degrees, -12 degrees ) treadmills to change the demand for mechanical work.

Force-velocity properties of two avian hindlimb muscles.

Recent work has provided measurements of power output in avian skeletal muscles during running and flying, but little is known about the contractile properties of avian skeletal muscle. We used an in situ preparation to characterize the force-velocity properties of two hind limb muscles, the lateral gastrocnemius (LG) and peroneus longus (PL), in Wild Turkeys (Meleagris gallopavo). A servomotor measured shortening velocity for at least six different loads over the plateau region of the length-tension curve.