Multiplexed measurement of proteins in tissue in a clinical environment.

There is an emerging need for more effective approaches to accurately quantitate protein expression in tissue samples. In many clinical studies and particularly in pharmaceutical clinical trials, access to adequate tissue samples is a major bottleneck, and thus techniques to measure protein expression in these valuable tissue specimens is important. This study will review current approaches in multiplexing of protein expression in tissue, and discusses new approaches using a novel image registration technique across multiple tissue sections.

The role and impact of quantitative discovery pathology.

The decision to advance an early-stage compound into formal preclinical testing depends on confidence in mechanism, efficacy and toxicity profiles. A substantial percentage of this confidence comes from histopathology interpretation, as the local tissue environment contains strong signals of both efficacy and toxicity. Accessing this tissue information is made difficult by biological variability across organs and tissues, an insufficient pool of pathology experts working in discovery, and the high subjectivity and individual isolation of microscope-based observations.