Serial passage of entomopathogenic Hyphomycetes has been shown to alter virulence and host specificity. We evaluated virulence, host specificity, biomass production, conidiation, conidial germination, and a genetic fingerprint of 3 strains of Paecilomyces fumosoroseus after passage in vitro or in vivo in Diuraphis noxia or Plutella xylostella. Strain 4461 did not change in virulence toward D.
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