Interplay between three RND efflux pumps in doxycycline-selected strains of Burkholderia thailandensis.

Background: Efflux systems are involved in multidrug resistance in most Gram-negative non-fermentative bacteria. We have chosen Burkholderia thailandensis to dissect the development of multidrug resistance phenotypes under antibiotic pressure.

Methodology/principal Findings: We used doxycycline selection to obtain several resistant B.