Publications by authors named "Francois Lieffrig"

Article Synopsis
  • * It was expanded to include two new families, 41 new genera, and 98 new species, along with reclassifications for 349 species.
  • * The article details the updated taxonomy of Negarnaviricota, including corrections of misspelled names for seven species.
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Perhabdoviruses are a threat to some freshwater fish species raised in aquaculture farms in Europe. Although the genetic diversity of these viruses is suspected to be high, the classification of isolates is still in its infancy, with just one full-length genome available and only partial sequences for a limited number of others. Here, we characterized a series of viruses isolated from percids in France from 1999 to 2009 by sequencing the nucleoprotein (N) gene.

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Article Synopsis
  • Piscine orthoreovirus genotype 1 (PRV-1) is commonly found in farmed Atlantic salmon in northern Europe and has been detected in wild fish in Norway and Canada, but its impact on wild populations is not well understood.
  • A survey conducted on 1,130 wild salmonids across various countries revealed the first instances of PRV-1 in wild fish from Denmark, Sweden, Faroe Islands, and Ireland, with prevalence rates varying by region.
  • The study highlights the need for monitoring PRV-1 in wild fish populations, especially broodfish for restocking programs, due to the virus's geographical spread and genetic diversity.
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In 2016, a total of 5 massive mortality episodes each affecting hundreds of thousands of pike-perch Sander lucioperca larvae occurred at 2 sites in 2 Western European countries. For each episode, perhabdoviruses related to the perch rhabdovirus (PRV) were detected in samples, using either PCR or cell culture combined with PCR. The sequences of the glycoprotein (g), phosphoprotein (p) and nucleoprotein (n) genes of these samples demonstrated that 2 different genotypes were present at 1 site, each associated with 1 of the 3 episodes.

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Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing a recombinant lacking open reading frame 134 (ORF134), we unexpectedly obtained a clone with additional deletion of ORF56 and ORF57.

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Cyprinid herpesvirus 3 (CyHV-3) causes a lethal disease in common and koi carp (Cyprinus carpio). The present study investigated the ability of CyHV-3 to infect common carp during the early stages of its development (from embryos to fingerlings) after inoculation by immersion in water containing the virus. Fish were inoculated at different times after hatching with a pathogenic recombinant CyHV-3 strain expressing luciferase.

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Cyprinid herpesvirus 3 (CyHV-3), also known as Koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. Recently, we investigated the entry of CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing CyHV-3.

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Article Synopsis
  • - Cyprinid herpesvirus 3 (CyHV-3) causes a deadly and highly contagious disease in common and koi carp, primarily entering through the skin when fish immerse in contaminated water.
  • - The study discovered that removing skin mucus and creating lesions enhanced the entry of CyHV-3, demonstrating that mucus acts as a barrier against the virus.
  • - Mucus extract was found to reduce the virus's infectivity in a lab setting, emphasizing the skin mucus's critical role in the fish's innate immune defense against viral infections.
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The recently designated cyprinid herpesvirus 3 (CyHV-3) is an emerging agent that causes fatal disease in common and koi carp. Since its emergence in the late 1990s, this highly contagious pathogen has caused severe financial losses in common and koi carp culture industries worldwide. In addition to its economic role, recent studies suggest that CyHV-3 may have a role in fundamental research.

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