Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain.

Flavoproteins of the quiescin/sulfhydryl oxidase (QSOX) family catalyze oxidation of peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. We report here the molecular cloning of a new putative sulfhydryl oxidase cDNA, rQSOX-L (GenBank Accession no ), from adult rat brain and its expression studied by RT-PCR, Northern and Western blots in rat tissues. DNA-sequencing demonstrated the existence of two cDNAs in rat cortex, corresponding to a long transcript (rQSOX-L) and a short transcript (rQSOX-S) which differed by 851 nucleotides due to alternative splicing.

Effect of sodium orthovanadate on glycosylation of the phosphopeptidomannans involved in the cell-cell aggregation of the yeast Kluyveromyces bulgaricus.

In studies of yeast flocculation it has been found that low concentrations of vanadium contained in sodium orthovanadate do not affect the growth and the cell-cell adhesion of the yeast Kluyveromyces bulgaricus, whereas high concentrations delay the growth of the yeasts and strongly inhibit flocculation. Moreover, higher sensitivity to Hygromycin B and calcofluor white was taken to imply altered cell wall integrity which is supported by compositional analysis of the extracted phosphopeptidomannans. Yeasts grown on sodium orthovanadate show a decrease in the percentage of phosphopeptidomannans and their compositions.