In Vitro Anti- Activity of Extracts Obtained from and : The Role of β-Amyrin.

Toxoplasmosis is a parasitic disease caused by the protozoan that is highly prevalent worldwide. Although the infection is asymptomatic in immunocompetent individuals, it severely affects immunocompromised individuals, causing conditions such as encephalitis, myocarditis, or pneumonitis. The limited therapeutic efficacy of drugs currently used to treat toxoplasmosis has prompted the search for new therapeutic alternatives.

Activation of the Keap1-Nrf2 pathway by specioside and the -butanol extract from the inner bark of (Bertol) DC.

A large number of chemical compounds exert their antioxidant effects by activation of key transcriptional regulatory mechanisms, such as the transcription factor Nrf2. The aim of this study was to evaluate the activation of the Keap1-Nrf2 pathway by both the -butanol extract obtained from the inner bark of (Bertol) DC and specioside isolated from this extract. The antioxidant activity of the extract and specioside isolated from the inner bark of were evaluated using the oxygen radical absorbance capacity (ORAC) and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) techniques, whereas their effects on the viability of HepG2 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.