Pharmacological inhibition reveals participation of the endocytic compartment in positive feedback IL-6 secretion in human skeletal myotubes.

IL-6 is an important cytokine involved in metabolic, immunological, and cell-fate responses. It is released upon stimulation by skeletal muscle cells through partially characterized mechanisms. In some cell types, IL-6 has been reported to activate a positive feedback loop involving endocytic vesicles, but evidence is mostly based on transcription and signal transduction mechanisms and is very scarce in muscle cells.

The first Neolithic boats in the Mediterranean: The settlement of La Marmotta (Anguillara Sabazia, Lazio, Italy).

Navigation in the Mediterranean in the Neolithic is studied here through the boats that were used, the degree of technical specialisation in their construction and, above all, their chronology. After a brief explanation of the exceptional site of La Marmotta, the characteristics and chronology of the five canoes found at the settlement and one of the nautical objects linked to Canoe 1 are discussed. This will allow a reflection on the capability of Neolithic societies for navigation owing to their high technological level.

Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from cf. blooms.

Analogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus . The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced toxins can be transferred through marine food webs. Thus, the determination of the concentration of PLTX analogues (ovatoxins-OVTXs, ostreocins-OSTs and isobaric PLTX) in different matrices (seawater, marine fauna, ) is necessary to protect human health.

Analysis of Dechlorane Plus and related compounds in gull eggs by GC-HRMS using a novel atmospheric pressure photoionization source.

Here, a new gas chromatography-atmospheric pressure photoionization-high-resolution mass spectrometry (GC-APPI-HRMS) method combined with selective pressurized liquid extraction (sPLE) has been developed for the selective determination of Dechlorane Plus (DP) and its related compounds in gull egg samples used as a bioindicator of contamination. To the best of our knowledge, this is the first time these compounds have been analyzed by GC-MS using atmospheric pressure photoionization (APPI). Negative ion dopant-assisted APPI using vapors of diethyl ether and a source temperature of 250 °C provided high ionization efficiencies and mass spectra characterized by intense in-source fragment ions as well as the presence of molecular ion and characteristic cluster ions containing oxygen atoms in their chemical structure.

Analysis of hydroxylated phenylalkylamine stimulants in urine by GC-APPI-HRMS.

A gas chromatography-atmospheric pressure photoionization-high-resolution mass spectrometry (GC-APPI-HRMS) method was developed for the determination of eight phenylalkylamine stimulants in urine samples. Spiked urine samples were hydrolyzed, processed by solid-phase extraction, and derivatized before analysis. Two derivatization reactions were studied: the formation of trimethylsilyl (TMS) derivatives with N-methyl-N-trimethylsilyl trifluoroacetamide (MSTFA) and trimethylsilyl/trifluoroacetyl (TMS/TFA) derivatives with MSTFA and N-methyl-bis (trifluoroacetamide) (MBTFA) as derivatization reagents.

Pigment profiles of Spanish extra virgin olive oils by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry.

This work studies the natural pigment profiles (chlorophylls and carotenoids) of Spanish Extra Virgin Olive Oils (EVOO) produced in different Spanish regions. The simultaneous qualitative and quantitative analysis of EVOO natural pigments has been performed by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) using atmospheric pressure chemical ionisation (APCI). The results showed a similar natural pigment pattern for all the analysed EVOOs, although the total pigments content differed significantly.

Age-Induced Differential Changes in the Central and Colonic Human Circadian Oscillators.

Aging modifies not only multiple cellular and homeostatic systems, but also biological rhythms. The circadian system is driven by a central hypothalamic oscillator which entrains peripheral oscillators, in both cases underlain by circadian genes. Our aim was to characterize the effect of aging in the circadian expression of clock genes in the human colon.

Presence and impact of Stockholm Convention POPs in gull eggs from Spanish and Portuguese natural and national parks.

The aim of the present work was to comparatively assess the occurrence and impact of persistent organic pollutants (POPs) in nine natural and national parks from Spain and Portugal using gull eggs (Larus michahellis and L. audouinii) as bioindicators of environmental contamination. Sampling was performed during the breeding season of 2016.

Radioguided surgery in primary hyperparathyroidism: Results and correlation with intraoperative histopathologic diagnosis.

Introduction And Objectives: Radioguided surgery is a minimally invasive surgical technique for the treatment of primary hyperparathyroidism. The goals of our study were to evaluate the rate of success and compare the results with intraoperative histological analysis.

Methods: We retrospectively studied 84 patients with primary parathyroidism who had undergone radioguided surgery.

Octodon degus, a new model to study the agonist and plexus-induced response in the urinary bladder.

Urinary bladder function consists in the storage and controlled voiding of urine. Translational studies require animal models that match human characteristics, such as Octodon degus, a diurnal rodent. This study aims to characterize the contractility of the detrusor muscle and the morphology and code of the vesical plexus from O.

In vitro effects of Pilocarpus microphyllus extracts and pilocarpine hydrochloride on Rhipicephalus (Boophilus) microplus.

The aim of this study was to assess the activity of aqueous (AE) and ethanolic extracts (EE) and pilocarpine hydrochloride, which were extracted and isolated from Pilocarpus microphyllus (Jaborandi), respectively, on Rhipicephalus (Boophilus) microplus. High performance liquid chromatography (HPLC) was performed to quantify these compounds. Larval packet and adult immersion tests were conducted with different concentrations.

Dioxins and dl-PCBs in gull eggs from Spanish Natural Parks (2010-2013).

The aim of this study was to evaluate the presence and distribution of polychlorinated dibenzo-p-dioxins, dibenzofurans (PCDD/Fs) and biphenyls (PCBs), concretely those so-called as dioxin-like PCBs, in yellow-legged gull eggs (Larus michahellis) collected from five Natural Parks (some of them National Parks) in Spain during the period 2010-2013. PCDD/Fs and dl-PCBs were detected in all the samples. Due to the proximity to important urban and industrial areas higher concentrations were determined in colonies located in the Northern Mediterranean coast than those found in the Southern Mediterranean or Atlantic colonies where a softer anthropogenic impact occurs.

Meteorologically-driven circulation and flushing times of the Bay of Algeciras, Strait of Gibraltar.

A primitive-equation model has been used to investigate the meteorologically-driven circulation of the Bay of Algeciras. It is shown that the mean circulation of Atlantic Water (AW) is characterized by an anticyclonic cell, while Mediterranean Water (MW) follows a preferred cyclonic pathway. Meteorological forcing distorts substantially the AW mean circulation pattern, and only modulates that of the MW.

Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant.

Ethnopharmacological Relevance: Bauhinia platypetala Burch. is a traditionally used Brazilian medicinal plant, although no evidence in the literature substantiates the safety of its use.

Aim Of The Study: The aim of this study was to investigate the safety of the ethanolic extract and the ethereal fraction of B.

Rhamnolipid emulsifying activity and emulsion stability: pH rules.

Rhamnolipids have excellent emulsifying power with a variety of hydrocarbons, aromatic compounds and vegetable oils. This paper evaluates the effect of pH ranging from 3 to 9 on the emulsifying activity and stability of rhamnolipids and hydrophobic substrates (benzene, soybean oil and kerosene). Sodium dodecyl sulfate was used as a reference to compare the level of chemical and biological surfactant activity after 24 h (E(24)).

Biomagnification of anthropogenic and naturally-produced organobrominated compounds in a marine food web from Sydney Harbour, Australia.

Polybrominated diphenyl ethers (PBDEs) and naturally-produced organobrominated compounds, such as methoxylated PBDEs (MeO-PBDEs), have been scarcely studied in the Southern Hemisphere. Yet, sources of the latter group of compounds were found in Southern regions, specifically in Australia. The environmental distribution and biomagnification potential of organobrominated compounds were therefore investigated in a representative aquatic food chain (invertebrates and fish) from the Sydney Harbour, Australia.

Anthropogenic and naturally occurring organobrominated compounds in two deep-sea fish species from the Mediterranean Sea.

Polybrominated diphenyl ethers (PBDEs) were investigated in 15 composite liver samples from two deep-sea fish species, the hollowsnout grenadier (Trachyrinchus trachyrinchus, TT) and the roughsnout grenadier (Coelorhynchus coelorynchus, CC). Mean concentrations of sum tri- to hepta-BDEs were higher in CC species than in the TT species (16.9 vs.